Strain Data Sheet

RBRC01519

Strain Information

Image
BRC No.RBRC01519
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Thy1-spH)42Yyan/Rbrc
Former Common nameTV-42, Thy1-SpH transgenic mice (TV-42)
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were crossed to C57BL/6J.
Strain descriptionVesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. Thy1-SpH transgenic mouse expresses SpH in brain under the control of neuron-specific Thy1.2 promoter. This strain is useful for studies of presynaptic terminals in various regions of the brain. The spH mRNAs was expressed in whole brains of TV-7, TV-10, and TV-34 transgenic lines, whereas in TV-42, it was expressed in the specific regions of the hippocampus. Tg mice mice show no obvious abnormality.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex.
Araki R, Sakagami H, Yanagawa Y, Hikima T, Ishizuka T, Yawo H
Genesis 42, 53-60 (2005). 15880564

beta-Phorbol ester-induced enhancement of exocytosis in large mossy fiber boutons of mouse hippocampus.
Hikima T, Araki R, Ishizuka T, Yawo H
J. Physiol. Sci., 59, 263-274 (2009). 19340534

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Vamp2
MGI:1313277		vesicle-associated membrane protein 2UNVamp2 Thy-1.2 promoter
  • neurodevelopmental disorder with hypotonia and autistic features with or without hyperkinetic movements(MedGEN)
    • pHluorin pH-sensitive Green Fluorescent Protein (Aequorea victoria)UNpHluorin

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmurine Thy-1.2 expression cassette (Caroni, P. J. Neurosci. Methods 71, 3-9,1997), mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis 42, 53-60 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Material should not be used for commercial purpose.
    DepositorHiromu Yawo (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2006

    BRC mice in Publications

    No Data