Strain Data Sheet

RBRC01527

Strain Information

Image
BRC No.RBRC01527
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(NP-IL2R/GFP)2-6Koba/KobaRbrc
Former Common nameNP-IL2R/2-6
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThe transgene contains mouse neuropsin gene promoter (BAC clone), human interleukin-2 receptor alpha-subunit/GFP. Subthalamic neurons are disrupted by a recombinant immunotoxin.
Colony maintenance
References
Subthalamic neurons coordinate basal ganglia function through differential neural pathways.
Yasoshima Y, Kai N, Yoshida S, Shiosaka S, Koyama Y, Kayama Y, Kobayashi K
J. Neurosci., 25, 7743-7753 (2005). 16120775

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN
IL2RA Interleukin-2 receptor α-subunit (human)UNIL2RA mouse neropsin (NP) promoter, rabbit beta-globin intron,rabbit beta-globin poly A, SV40 early gene poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal locomotor behavior(MP:0001392)

  • abnormal synaptic glutamate release(MP:0004494)

  • decreased locomotor activity(MP:0001402)

  • impaired ability to fire action potentials(MP:0002578)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse neropsin (NP) promoter, rabbit beta-globin intron, human Interleukin-2 receptor α-subunit cDNA, rabbit beta-globin poly A, SV40 early gene poly A, jellyfish GFP cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 25 (34) 7743-7753 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data