Strain Data Sheet

RBRC01527

Strain Information

Image
BRC No.RBRC01527
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(NP-IL2R/GFP)2-6Koba/KobaRbrc
Former Common nameNP-IL2R/2-6
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThe transgene contains mouse neuropsin gene promoter (BAC clone), human interleukin-2 receptor alpha-subunit/GFP. Subthalamic neurons are disrupted by a recombinant immunotoxin.
Colony maintenance
ReferencesJ. Neurosci., 25, 7743-7753 (2005). 16120775

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IL2RAInterleukin-2 receptor α-subunit (human)UNIL2RAmouse neropsin (NP) promoter, rabbit beta-globin intron,rabbit beta-globin poly A, SV40 early gene poly A

Ordering Information

Donor DNAmouse neropsin (NP) promoter, rabbit beta-globin intron, human Interleukin-2 receptor α-subunit cDNA, rabbit beta-globin poly A, SV40 early gene poly A, jellyfish GFP cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 25 (34) 7743-7753 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data