Strain Data Sheet

RBRC01543

Strain Information

Image
BRC No.RBRC01543
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Foxn3<Tn(sb-lacZ,GFP)PV321Jtak>/JtakRbrc
Former Common nameGene trap of Ches1 (checkpoint suppressor 1), PV321
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
BCL2 Splice acceptor (human bcl-2)12BCL2
Ches1
MGI:1918625		checkpoint suppressor 112Ches1<Tn(sb-lacZ,GFP)PV321Jtak> transposon insertion PV321, Junji Takeda
GFP Green Fluorescent Protein (Aequorea victoria)12 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
IRES internal ribosomal entry site (EMCV)12
Splice donor Splice donor (mouse hprt)12Splice donor
lacZ beta-galactosidase (E. coli)12
neo neomycin resistance gene (E. coli)12
poly A polyA12poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, SD, SA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data