Strain Data Sheet

RBRC01544

Strain Information

Image
BRC No.RBRC01544
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Sirpa<tm1Nog>/Rbrc
Former Common nameSHPS-1 Knockout mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NJcl
Appearanceblack
Strain developmentDeveloped by Dr. Kenjiro Inagaki, Kobe University School of Medicine in 2000. The construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed C57BL/6N over 10 times.
Strain descriptionSHPS-1 knockout mice. Exon 7 and 8 were replaced with a nemoycin selection cassette. SHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene ia a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.
References
SHPS-1 regulates integrin-mediated cytoskeletal reorganization and cell motility.
Inagaki K, Yamao T, Noguchi T, Matozaki T, Fukunaga K, Takada T, Hosooka T, Akira S, Kasuga M
EMBO J., 19, 6721-6731 (2000). 11118207

Negative regulation of platelet clearance and of the macrophage phagocytic response by the transmembrane glycoprotein SHPS-1.
Takuji Yamao, Tetsuya Noguchi, Osamu Takeuchi, Uichi Nishiyama, Haruhiko Morita, Tetsuya Hagiwara, Hironori Akahori, Takashi Kato, Kenjiro Inagaki, Hideki Okazawa, Yoshitake Hayashi, Takashi Matozaki, Kiyoshi Takeda, Shizuo Akira, Masato Kasuga
J. Biol. Chem., 277 (42) 39833-9 (2002). 12167615

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sirpa
MGI:108563		signal-regulatory protein alpha2Sirpa<tm1Nog>
MGI:2388979		targeted mutation 1, Tetsuya Noguchi
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Langerhans cell physiology(MP:0005362)

  • abnormal macrophage physiology(MP:0002451)

  • behavioral despair(MP:0002573)

  • decreased T cell number(MP:0005018)

  • decreased body weight(MP:0001262)
    • more 3 phenotypes
  • hepatic steatosis(MP:0002628)

  • increased megakaryocyte cell number(MP:0008254)

  • thrombocytopenia(MP:0003179)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sirpa genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must submit its receipt to the DEPOSITOR immediately after receiving the BIOLOGICAL RESOURCE.
    DepositorTakashi Matozaki (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Christina Wenzek, Philine Steinbach, Florian Wirsdörfer, Kathrin Sutter, Julia D. Boehme, Robert Geffers, Robert Klopfleisch, Dunja Bruder, Verena Jendrossek, Jan Buer, Astrid M. Westendorf, Torben Knuschke
    CD47 restricts antiviral function of alveolar macrophages during influenza virus infection
    iScience 25 105540(2022) 36437873
    Sarute N, Cheng H, Yan Z, Salas-Briceno K, Richner J, Rong L, Ross SR.
    Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways.
    PLoS Pathog 17(6) e1009662(2021) 34097709
    Cham LB, Torrez Dulgeroff LB, Tal MC, Adomati T, Li F, Bhat H, Huang A, Lang PA, Moreno ME, Rivera JM, Galkina SA, Kosikova G, Stoddart CA, McCune JM, Myers LM, Weissman IL, Lang KS, Hasenkrug KJ.
    Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection.
    Cell Rep 31(2) 107494(2020) 32294445
    Li LX, Atif SM, Schmiel SE, Lee SJ, McSorley SJ.
    Increased susceptibility to Salmonella infection in signal regulatory protein α-deficient mice.
    J Immunol 189(5) 2537-44(2012) 22851710
    Wang L, Lu Y, Deng S, Zhang Y, Yang L, Guan Y, Matozaki T, Ohnishi H, Jiang H, Li H.
    SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress.
    J Neurochem 122(4) 834-43(2012) 22671569
    Mark Kearns, William J. Janssen, and Peter M. Henson
    Signal Inhibitory Regulatory Protein Alpha Influences Macrophage Clearance From The Alveolar Space After An Inflammatory Stimulus.
    Am. J. Respir. Crit. Care Med. 181 A2761(2010)