Strain Data Sheet

RBRC01558

Strain Information

Image
BRC No.RBRC01558
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nrxn3<Tn(sb-lacZ,GFP)OR5Jtak> Nrxn1<Tn(sb-lacZ,GFP)OR85Jtak>/JtakRbrc
Former Common nameOR5/85
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
BCL2 Splice acceptor (human bcl-2)12BCL2
GFP Green Fluorescent Protein (Aequorea victoria)12 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
IRES internal ribosomal entry site (EMCV)12
Nrxn3
MGI:1096389		neurexin III12Nrxn3<Tn(sb-lacZ,GFP)OR5Jtak> transposon insertion OR5, Junji Takeda
Splice donor Splice donor (mouse hprt)12Splice donor
lacZ beta-galactosidase (E. coli)12
BCL2 Splice acceptor (human bcl-2)17BCL2
GFP Green Fluorescent Protein (Aequorea victoria)17 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
IRES internal ribosomal entry site (EMCV)17
Nrxn1
MGI:1096391		neurexin I17Nrxn1<Tn(sb-lacZ,GFP)OR85Jtak> transposon insertion OR85, Junji Takeda
  • chromosome 2p16.3 deletion syndrome(MedGEN)

  • Pitt-Hopkins-like syndrome 2(MedGEN)
    • Splice donor Splice donor (mouse hprt)17Splice donor
    lacZ beta-galactosidase (E. coli)17

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNApTrans-SA, Encephalomyocarditis virus (EMCV) internal ribosomal entrysite (ires), E. coli lacZ, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish EGFP cDNA, SD, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data