Strain Data Sheet

RBRC01652

Strain Information

Image
BRC No.RBRC01652
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameIF2
Former Common nameSB transposon IF2
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack
Strain developmentDeveloped by Drs. Kyoji Horie and Junji Takeda at Osaka University in 2004. The SB transposon vecotor consists of a GFP expression cassette flanked by a splice donor and a lacZ cassette flanked by acceptor, allowing for poly (A)-trap selection of transposition events and promoter-trap for endogenous gene expression pattern profiling , respectively. This transposon vector was injected into the pronuclei of B6D2F2 fertilized egges. C57BL/6, DBA/2 and ICR mixed background.
Strain descriptionThis transgenic mouse strain has a Sleeping Beauty (SB) transposon vector acting as a mutagen. The double transgenic mice containing SB transposon vector and SB transposase, generated by crossing with this Tg mice and the Tg mice having SB transposase, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6J)
References
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
IRES internal ribosomal entry site (EMCV)UN
SA Splice acceptorUNSA
Splice donor Splice donorUNSplice donor
lacZ beta-galactosidase (E. coli)UN
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
neo neomycin resistance gene (E. coli)UN mouse phosphoglycerate kinase promoter (PGK promoter)
nls SV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNApTrans-SA-IRESLacZ-CAG-GFP-SD:Neo[SA (The human bcl-2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, Simian virus 40 Large T antigen nuclear localization signal (nls), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), EGFP, SD(splice donor sequences from the mouse hprt gene), E.coli NEO, the mRNA instability signal derived from the 3′ untranslated region of the human granulocyte-macrophage colony-stimulating factor cDNA], mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
    Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
    Exp Anim 58(4) 437-42(2009) 19654444