Strain Data Sheet
RBRC01680
Strain Information | |
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| Image |  |
| BRC No. | RBRC01680 |
| Type | Gene Trap |
| Species | Mus musculus |
| Strain name | B6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc |
| Former Common name | Gene trap of Smoc1 (SPARC related modular calcium binding 1), PV384 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Junji Takeda at Osaka University in 2005. |
| Strain description | This strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system. |
| Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
| References | Characterization of Sleeping Beauty transposition and its application to genetic screening in mice. Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system. Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J Nat. Methods, 2, 763-769 (2005). 16179923 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| BCL2 | Splice acceptor (human bcl-2) | 12 | BCL2 | ||||
| GFP | Green Fluorescent Protein (Aequorea victoria) | 12 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | ||||
| GH | Growth hormone polyA (human) | 12 | GH | ||||
| HBB2 | rabbit beta-globin polyA | 12 | HBB2 | ||||
| IRES | internal ribosomal entry site (EMCV) | 12 | |||||
| Pgk1 | mouse pgk promoter | 12 | Pgk1 | ||||
| SA | Splice acceptor (Salmon) | 12 | SA | ||||
| Smoc1 MGI:1929878 | SPARC related modular calcium binding 1 | 12 | Smoc1<Tn(sb-lacZ,GFP)PV384Jtak> MGI:4941776 | gene trap lacZ/GFP PV384, Junji Takeda | |||
| Splice donor | Splice donor (mouse hprt) | 12 | Splice donor | ||||
| Splice donor | Sleeping Beauty transposase | 12 | Splice donor | ||||
| lacZ | beta-galactosidase (E. coli) | 12 | |||||
| neo | neomycin resistance gene (E. coli) | 12 | |||||
| mouse synaptonemal protein 1 promoter | |||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 20 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Salmon Sleeping Beauty transposon, Salmon Sleeping Beauty transposase, Jellyfish GFP gene, E. coli LacZ gene, E. coli neo gene, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human bcl-2 splice acceptor, Mouse hprt splice donor, Cytomegalovirus enhancer, Chicken beta-actin promoter, Mouse pgk promoter, Rabbit beta-globin polyadenylation signal sequence, Mouse synaptonemal protein 1 promoter, Human growth hormone polyadenylation signal sequence, pBluescript |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Junji Takeda (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Xiaoming Li, Sebastian Kempf, Fredy Delgado Lagos, Ürün Ukan, Rüdiger Popp, Jiong Hu, Timo Frömel, Stefan Günther, Andreas Weigert, Ingrid Fleming A regulatory loop involving the cytochrome P450-soluble epoxide hydrolase axis and TGF-β signaling iScience 110938(2024) |
Delgado Lagos F, Elgheznawy A, Kyselova A, Meyer Zu Heringdorf D, Ratiu C, Randriamboavonjy V, Mann AW, Fisslthaler B, Siragusa M, Fleming I. Secreted modular calcium-binding protein 1 binds and activates thrombin to account for platelet hyperreactivity in diabetes. Blood 137(12) 1641-1651(2021) 33529332 |
Awwad K, Hu J, Shi L, Mangels N, Abdel Malik R, Zippel N, Fisslthaler B, Eble JA, Pfeilschifter J, Popp R, Fleming I. Role of secreted modular calcium-binding protein 1 (SMOC1) in transforming growth factor β signalling and angiogenesis. Cardiovasc Res 106(2) 284-94(2015) 25750188 |
Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H. SMOC1 is essential for ocular and limb development in humans and mice. Am J Hum Genet 88(1) 30-41(2011) 21194678 |