Strain Information | |
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Image | ![]() |
BRC No. | RBRC01725 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129-Dnase2a<tm1Osa>/OsaRbrc |
Former Common name | DNase II Hetero; DNase II Hetero Knockout mouse |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2001. A neomycin cassette replaced exons 1-5 and part of 6 of the Dnase2a gene. The mutant mice were backcrossed to C57BL/6. |
Strain description | B6;129-Dnase2a<tm1Osa>. In macrophage, DNase II, deoxyribonuclease II is responsible for digesting nuclear DNA expelled from erythroid precuursor cells. Homozygous null mice for DNase II are embryonic lethal due to sever anemia, and impaired definitive erythropoiesis in the fetal liver. This strain is useful for elucidation of the function of DNase II gene. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) |
References | Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver. Kawane K, Fukuyama H, Kondoh G, Takeda J, Ohsawa Y, Uchiyama Y, Nagata S Science, 292, 1546-1549 (2001). 11375492 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Dnase2a MGI:1329019 | deoxyribonuclease II alpha | 8 | Dnase2a<tm1Osa> MGI:2449811 | targeted mutation 1, Shigekazu Nagata | |||
loxP | phage P1 loxP | 8 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 8 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 10 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Dnase2a genomic DNA, Phage P1 loxP site [update May 21, 2015] |
Research application | Mouse Models for Human Disease |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 292, 1546-1549 (2001). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author. |
Depositor | Shigekazu Nagata (Kyoto University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Chan MP, Onji M, Fukui R, Kawane K, Shibata T, Saitoh S, Ohto U, Shimizu T, Barber GN, Miyake K. DNase II-dependent DNA digestion is required for DNA sensing by TLR9. Nat Commun 6 5853(2015) 25600358 |
Ahn J, Gutman D, Saijo S, Barber GN. STING manifests self DNA-dependent inflammatory disease. Proc Natl Acad Sci U S A 109(47) 19386-91(2012) 23132945 |