Strain Data Sheet

RBRC01725

Strain Information

Image
BRC No.RBRC01725
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Dnase2a<tm1Osa>/OsaRbrc
Former Common nameDNase II Hetero; DNase II Hetero Knockout mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2001. A neomycin cassette replaced exons 1-5 and part of 6 of the Dnase2a gene. The mutant mice were backcrossed to C57BL/6.
Strain descriptionB6;129-Dnase2a<tm1Osa>. In macrophage, DNase II, deoxyribonuclease II is responsible for digesting nuclear DNA expelled from erythroid precuursor cells. Homozygous null mice for DNase II are embryonic lethal due to sever anemia, and impaired definitive erythropoiesis in the fetal liver. This strain is useful for elucidation of the function of DNase II gene.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
References
Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver.
Kawane K, Fukuyama H, Kondoh G, Takeda J, Ohsawa Y, Uchiyama Y, Nagata S
Science, 292, 1546-1549 (2001). 11375492

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnase2a
MGI:1329019
deoxyribonuclease II alpha8Dnase2a<tm1Osa>
MGI:2449811
targeted mutation 1, Shigekazu Nagata
loxP phage P1 loxP8loxP
neo neomycin resistance gene (E. coli)8 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal blood homeostasis(MP:0009642)

  • abnormal cytokine level(MP:0008713)

  • abnormal erythropoiesis(MP:0000245)

  • abnormal macrophage morphology(MP:0002446)

  • abnormal thymus morphology(MP:0000703)
  • more 10 phenotypes
  • anemia(MP:0001577)

  • decreased erythrocyte cell number(MP:0002875)

  • increased circulating tumor necrosis factor level(MP:0008553)

  • increased nucleated erythrocyte cell number(MP:0009395)

  • joint inflammation(MP:0002933)

  • lethality during fetal growth through weaning, complete penetrance(MP:0011111)

  • postnatal growth retardation(MP:0001732)

  • rheumatoid arthritis(MP:0003561)

  • small thymus(MP:0000706)

  • thymus hypoplasia(MP:0001823)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Dnase2a genomic DNA, Phage P1 loxP site [update May 21, 2015]
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 292, 1546-1549 (2001). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
    The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
    DepositorShigekazu Nagata (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Chan MP, Onji M, Fukui R, Kawane K, Shibata T, Saitoh S, Ohto U, Shimizu T, Barber GN, Miyake K.
    DNase II-dependent DNA digestion is required for DNA sensing by TLR9.
    Nat Commun 6 5853(2015) 25600358
    Ahn J, Gutman D, Saijo S, Barber GN.
    STING manifests self DNA-dependent inflammatory disease.
    Proc Natl Acad Sci U S A 109(47) 19386-91(2012) 23132945