Strain Data Sheet

RBRC01733

Strain Information

Image
BRC No.RBRC01733
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ptafr<tm1Tksh>/TkshRbrc
Former Common namePAFR-KO, Platelet-activating factor (PAF) receptor knockout mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Satoshi Ishii at the University of Tokyo in 1998. A neomycin cassette was inserted into the open reading frame of Ptafr gene. The mutant mice were backcrossed to C57BL/6.
Strain descriptionPlatelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. Homozygous mutant mice showed decreased susceptibility to bacterial infection. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
References
Impaired anaphylactic responses with intact sensitivity to endotoxin in mice lacking a platelet-activating factor receptor.
Ishii S, Kuwaki T, Nagase T, Maki K, Tashiro F, Sunaga S, Cao W H, Kume K, Fukuchi Y, Ikuta K, Miyazaki J, Kumada M, Shimizu T
J. Exp. Med., 187, 1779-1788 (1998). 9607919

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ptafr
MGI:106066		platelet-activating factor receptor4Ptafr<tm1Tksh>
MGI:3036770		targeted mutation 1, Takao Shimizu
neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • cardiovascular system phenotype(MP:0005385)

  • decreased inflammatory response(MP:0001876)

  • decreased susceptibility to bacterial infection(MP:0002411)

  • increased susceptibility to parasitic infection(MP:0005027)

  • increased susceptibility to type I hypersensitivity reaction(MP:0005596)
    • more 2 phenotypes
  • nervous system phenotype(MP:0003631)

  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk polyA, mouse platelet-activating factor receptor (PAF) genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 187, 1779-1788 (1998).
    DepositorTakao Shimizu (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Kim BK, Shin EJ, Kim HC, Chung YH, Dang DK, Jung BD, Park DH, Wie MB, Kim WK, Shimizu T, Nabeshima T, Jeong JH.
    Platelet-activating factor receptor knockout mice are protected from MPTP-induced dopaminergic degeneration.
    Neurochem Int 63(3) 121-32(2013) 23743065
    Tanaka Y, Nagai Y, Kuroishi T, Endo Y, Sugawara S.
    Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock.
    J Leukoc Biol 91(3) 485-94(2012) 22131343