Strain Data Sheet

RBRC01765

Strain Information

Image
BRC No.RBRC01765
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S4-Tob1<tm1Tya>/TyaRbrc
Former Common nametob-gene KO (B6)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Tetsuo Noda at Japanese Foundation for Cancer Research Cancer Institute and Dr. Tadashi Yamamoto at the University of Tokyo in 1997. A PGK-neo cassette was inserted into the cording region of Tob1 gene. C57BL/6J background.
Strain descriptionTob is a member of antiproliferative protein family which is a negative regulator BMP/Smad signaling in osteoblasts. Tob1 null mice are viable and exhibit bone mass due to increased numbers of osteoblasts. This strain is useful for elucidating of Tob gene function.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Negative regulation of BMP/Smad signaling by Tob in osteoblasts.
Yoshida Y, Tanaka S, Umemori H, Minowa O, Usui M, Ikematsu N, Hosoda E, Imamura T, Kuno J, Yamashita T, Miyazono K, Noda M, Noda T, Yamamoto T
Cell, 103, 1085-1097 (2000). 11163184

Mice lacking a transcriptional corepressor Tob are predisposed to cancer.
Yoshida Y, Nakamura T, Komoda M, Satoh H, Suzuki T, Tsuzuku J K, Miyasaka T, Yoshida E H, Umemori H, Kunisaki R K, Tani K, Ishii S, Mori S, Suganuma M, Noda T, Yamamoto T
Genes Dev., 17, 1201-1206 (2003). 12756225

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tob1
MGI:1349721		transducer of ErbB-2.111Tob1<tm1Tya>
MGI:1933294		targeted mutation 1, Tadashi Yamamoto
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone ossification(MP:0008271)

  • abnormal osteoblast differentiation(MP:0008395)

  • increased bone mass(MP:0005605)

  • increased cell proliferation(MP:0000351)

  • increased osteoblast cell number(MP:0004988)
    • more 1 phenotypes
  • increased trabecular bone mass(MP:0010872)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse tob genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorTadashi Yamamoto (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Kitajima Y, Yoshioka K, Mikumo Y, Ohki S, Maehara K, Ohkawa Y, Ono Y.
    Loss of Tob1 promotes muscle regeneration through muscle stem cell expansion.
    J Cell Sci 137(15) (2024) 39037211
    Didonna A, Cekanaviciute E, Oksenberg JR, Baranzini SE.
    Immune cell-specific transcriptional profiling highlights distinct molecular pathways controlled by Tob1 upon experimental autoimmune encephalomyelitis.
    Sci Rep 6 31603(2016) 27546286
    Schulze-Topphoff U, Casazza S, Varrin-Doyer M, Pekarek K, Sobel RA, Hauser SL, Oksenberg JR, Zamvil SS, Baranzini SE.
    Tob1 plays a critical role in the activation of encephalitogenic T cells in CNS autoimmunity.
    J Exp Med 210(7) 1301-9(2013) 23797093