Strain Data Sheet

RBRC01774

Strain Information

Image
BRC No.RBRC01774
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ep400<tm1Fuku>/FukuRbrc
Former Common namemDom-KO, mDomino knockout mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Rikiro Fukunaga at Osaka University in 2003. Part of exon 2 and inron 2 was replaced with a neomycin selection cassette. The mutant mice were backcrossed to C57BL/6.
Strain descriptionB6.129-Ep400<tm1Fuku>. p400/mDomino (mammalian Domino) gene is an Swr1-class DNA-dependent ATPase that is a core subunit of a large chromatin-remodeling complex, and is currently suggested to play a unique function in histone variant exchange. The mDomino deficient mice are embryonic lethal and show anemic in appearance and slight deformity of the neural tubes.
Colony maintenanceBackcross to C57BL/6 Backcross to C57BL/6 (Heterozygote x C57BL/6N)
References
Critical role of the p400/mDomino chromatin-remodeling ATPase in embryonic hematopoiesis.
Ueda T, Watanabe-Fukunaga R, Ogawa H, Fukuyama H, Higashi Y, Nagata S, Fukunaga R
Genes Cells, 12, 581-592 (2007). 17535249

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ep400
MGI:1276124		E1A binding protein p4005Ep400<tm1Fuku> targeted mutation 1, Rikiro Fukunaga
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal common myeloid progenitor cell morphology(MP:0006410)

  • abnormal neural tube morphology(MP:0002151)

  • absent erythrocytes(MP:0000215)

  • anemia(MP:0001577)

  • embryonic growth arrest(MP:0001730)
    • more 2 phenotypes
  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • wavy neural tube(MP:0000930)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase-1 promoter, E. coli neo, mouse mDomino genomic DNA
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells, 12, 581-592 (2007). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
    The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
    DepositorRikiro Fukunaga (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Jan 2008

    BRC mice in Publications

    Chevillard-Briet M, Quaranta M, Grézy A, Mattera L, Courilleau C, Philippe M, Mercier P, Corpet D, Lough J, Ueda T, Fukunaga R, Trouche D, Escaffit F.
    Interplay between chromatin-modifying enzymes controls colon cancer progression through Wnt signaling.
    Hum Mol Genet 23(8) 2120-31(2014) 24287617