Strain Data Sheet

RBRC01840

Strain Information

Image
BRC No.RBRC01840
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Grin2d<tm1Mim>/MimRbrc
Former Common nameGluRε4 KO mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6
Appearanceblack
Strain developmentDeveloped by Kazutaka Ikeda and Masami Mishina, Brain Research Institute, Niigata University in 1995. A pgk-neo was transfered into TT2 ES cells to replace the exon encoding the M4 putative transmembrane segment of Grin2d gene. The mutant mice were backcrossed to C57BL/6N over 10 times.
Strain descriptionGluR epsilon4 (Grin2d, glutamate receptor, ionotropic, NMDA2D (epsilon 4)) knockout mice. The Grin2d gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel. The Grin2d homozygous mutant mice show a reduced spontaneous activity, but no significant difference in motor activity and anxiety tests when compared with normal mice. Reproductive performance and growth of them is normal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.
References
Reduced spontaneous activity of mice defective in the epsilon 4 subunit of the NMDA receptor channel.
Ikeda K, Araki K, Takayama C, Inoue Y, Yagi T, Aizawa S, Mishina M
Mol. Brain Res., 33, 61-71 (1995). 8774946

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grin2d
MGI:95823		glutamate receptor, ionotropic, NMDA2D (epsilon 4)7Grin2d<tm1Mim>
MGI:1928298		targeted mutation 1, Masayoshi Mishina
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • behavior/neurological phenotype(MP:0005386)

  • decreased locomotor activity(MP:0001402)

  • decreased vertical activity(MP:0002757)

  • increased startle reflex(MP:0001488)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse GluRε4 subunit genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain. Res., 33, 61-71 (1995). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
    DepositorMasayoshi Mishina (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Doyle MA, Salimando GJ, Altemus ME, Badt JK, Bedenbaugh MN, Vardy AS, Adank DN, Park AS, Winder DG.
    BNST GluN2D-containing NMDARs contribute to ethanol intake but not negative affective behaviors in female mice.
    Alcohol Clin Exp Res (Hoboken) 48(10) 1876-1891(2024) 39179522
    Sharma R, Neupane C, Pham TL, Lee M, Lee S, Lee SY, Nam MH, Kim CS, Park JB.
    Tonic Activation of NR2D-Containing NMDARs Exacerbates Dopaminergic Neuronal Loss in MPTP-Injected Parkinsonian Mice.
    J Neurosci 43(46) 7730-7744(2023) 37726169
    Doyle MA, Brown JA, Winder DG.
    GluN2D expression is regulated by restraint stress and supports active stress coping bouts.
    Neuropharmacology 225 109377(2023) 36528117
    Neupane C, Sharma R, Pai YH, Lee SY, Jeon BH, Kim HW, Stern JE, Park JB.
    High Salt Intake Recruits Tonic Activation of NR2D Subunit-Containing Extrasynaptic NMDARs in Vasopressin Neurons.
    J Neurosci 41(6) 1145-1156(2021) 33303677
    Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.
    Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: glutamate N2A/B/D [GluRε1/2/4] subtypes and the GABA synthesizing enzyme GAD65.
    Neuroscience 250 743-54(2013) 23892010
    Obiang P, Macrez R, Jullienne A, Bertrand T, Lesept F, Ali C, Maubert E, Vivien D, Agin V.
    GluN2D subunit-containing NMDA receptors control tissue plasminogen activator-mediated spatial memory.
    J Neurosci 32(37) 12726-34(2012) 22972996