Strain Information | |
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Image | |
BRC No. | RBRC01872 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | FVB;129P2(Cg)-Apc<tm2.1Cip>/GvnRbrc |
Former Common name | ApcFlox14 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | albino [A/A B/B c/c D/D P/P] |
Strain development | Developed by Dr. Marco Giovannini, INSRM France. FVB/N background. |
Strain description | FVB.Cg-Apc<tm2.1Cip>. Apc, adenomatosis polyposis coli is a member of the Wnt signaling pathway that is involved in development and tumorigenesis. This conditional knockout mice have a loxP-flanked Apc allele. Conditional Apc deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Sibling Mating |
References | Lab Invest., 84, 1619-1930 (2004). 15502862 Proc. Natl. Acad. Sci. USA, 101, 17216-17221 (2004). 15563600 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, mouse Apc genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci., 101, 17216-17221 (2004). Lab Invest., 84, 1619-1930 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT shall keep DEPOSITOR apprised of any results obtained from the BIOLOGICAL RESOURCE in the course of the research, as soon as they are available. Moreover, if the research results in a potential invention or a substance that may have potential commercial value (hereinafter referred as to the "Invention") RECIPIENT will promptly disclose this Invention to DEPOSITOR with samples thereof for DEPOSITOR's internal use. RECIPIENT and DEPOSITOR will consult together and agree about the possibility of filing one or more patent application to Invention conceived by one or more employees of Recipients that directly arise out of the research. The Parties will negotiate in good faith the conditions of such patent application according to each party's inventiveness contribution in the Invention. In case of such Invention, patentable or not, reduced in practice or developed in the course of the research that could not have been obtained, reduced to practice or developed but from using the Material and/or Confidential Information, RECIPIENT and DEPOSITOR shall confer with one another as regards licensing and commercialization of such Invention to negotiate in good faith the terms of an institutional agreement as regards said matters. |
Depositor | Marco Giovannini (INSERM) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Nakata T, Shimizu H, Nagata S, Ito G, Fujii S, Suzuki K, Kawamoto A, Ishibashi F, Kuno R, Anzai S, Murano T, Mizutani T, Oshima S, Tsuchiya K, Nakamura T, Hozumi K, Watanabe M, Okamoto R. Indispensable role of Notch ligand-dependent signaling in the proliferation and stem cell niche maintenance of APC-deficient intestinal tumors. Biochem Biophys Res Commun 482(4) 1296-1303(2017) 27939883 Funato Y, Yamazaki D, Mizukami S, Du L, Kikuchi K, Miki H. Membrane protein CNNM4-dependent Mg2+ efflux suppresses tumor progression. J Clin Invest 124(12) 5398-410(2014) 25347473 Ishibashi F, Shimizu H, Nakata T, Fujii S, Suzuki K, Kawamoto A, Anzai S, Kuno R, Nagata S, Ito G, Murano T, Mizutani T, Oshima S, Tsuchiya K, Nakamura T, Watanabe M, Okamoto R. Contribution of ATOH1+ Cells to the Homeostasis, Repair, and Tumorigenesis of the Colonic Epithelium. Stem Cell Reports 10(1) 27-42(2018) 29233556 |