Strain Data Sheet

RBRC01888

Strain Information

Image
BRC No.RBRC01888
TypeSpontaneous Mutation Congenic
SpeciesMus musculus
Strain nameC57BL/6-W<sh>/W<sh>
Former Common nameW sash, C57BL/6-W<sh>
H-2 Haplotype
ES Cell line
Background strainC57BL/6J
Appearanceblack with white spotting [a/a B/B C/C Sh/+]
black [a/a B/B C/C +/+]
white, black eyes [a/a B/B C/C Sh/Sh]
Strain developmentDeveloped by Keisuke Oboki, RIKEN Research Center for Allergy anf Immunology. This strain was generated by backcrossing to C57BL/6 from C;B6-a/a-b/b-W<sh>/W<sh> strain (RBRC00762).
Strain descriptionThe Kit<W-sh> mutation arose spontaneously in the (C3H/HeN x 101/H)F1 at the MRC (Lyon et al., 1982). Homozygous mice are white coat with black eyes. Heterozygotes are black with a white sash. This strain is useful for study of melanogenesis and neural crest migration.
Colony maintenanceHeteryozygote x Homozygote. Homozygous mutant mice are viable and fertile.
References
A new allele sash (Wsh) at the W-locus and a spontaneous recessive lethal in mice.
Lyon M F, Glenister P H
Genet. Res., 39, 315-322 (1982). 7117838

c-kit Gene was not transcribed in cultured mast cells of mast cell-deficient Wsh/Wsh mice that have a normal number of erythrocytes and a normal c-kit coding region.
Tono T, Tsujimura T, Koshimizu U, Kasugai T, Adachi S, Isozaki K, Nishikawa S, Morimoto M, Nishimune Y, Nomura S, et a l
Blood, 80, 1448-1453 (1992). 1381627

W-sash affects positive and negative elements controlling c-kit expression: ectopic c-kit expression at sites of kit-ligand expression affects melanogenesis.
Duttlinger R, Manova K, Chu T Y, Gyssler C, Zelenetz A D, Bachvarova R F, Besmer P
Development, 118, 705-717 (1993). 7521281

C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression.
Yamazaki M, Tsujimura T, Morii E, Isozaki K, Onoue H, Nomura S, Kitamura Y
Blood, 83, 3509-3516 (1994). 7515715

The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.
Berrozpe G, Timokhina I, Yukl S, Tajima Y, Ono M, Zelenetz A D, Besmer P
Blood, 94, 2658-2666 (1999). 10515869

Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Grimbaldeston M A, Chen C C, Piliponsky A M, Tsai M, Tam S Y, Galli S J
Am. J. Pathal., 167, 835-848 (2005). 16127161

Health Report

Examination Date / Room / Rack2024/12/09Room:3-6Rack:ASentinel mouse program
2024/09/09Room:3-6Rack:ASentinel mouse program
2024/06/10Room:3-6Rack:ASentinel mouse program
2024/03/11Room:3-6Rack:ASentinel mouse program
2023/12/11Room:3-6Rack:ASentinel mouse program
2023/09/11Room:3-6Rack:ASentinel mouse program
2023/07/18Room:3-6Rack:AAdditional tests
2023/06/13Room:3-6Rack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kit
MGI:96677		KIT proto-oncogene receptor tyrosine kinase5Kit<W-Sh>
MGI:1856265		sash
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
    • more 3 Diseases
  • mastocytosis(DisGeNET)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal coat/hair pigmentation(MP:0002075)

  • abnormal definitive hematopoiesis(MP:0002123)

  • abnormal erythrocyte cell number(MP:0001586)

  • abnormal melanogenesis(MP:0005077)

  • absent coat pigmentation(MP:0005171)
    • more 6 phenotypes
  • decreased erythrocyte cell number(MP:0002875)

  • decreased mast cell degranulation(MP:0008765)

  • decreased mast cell number(MP:0000336)

  • impaired behavioral response to xenobiotic(MP:0009747)

  • increased susceptibility to parasitic infection(MP:0005027)

  • variable body spotting(MP:0002940)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationDermatology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorKeisuke Oboki (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Yoshida K, Tanihara S, Miyashita Y, Obayashi K, Ito MA, Yamamoto K, Imai T, Matsuoka I.
    P2X4 receptor stimulation enhances MrgprB2-mediated mast cell activation and pseudoallergic reactions in mice.
    Sci Rep 12(1) 18613(2022) 36329102
    Murata A, Hikosaka M, Yoshino M, Zhou L, Hayashi SI.
    Kit-independent mast cell adhesion mediated by Notch.
    Int Immunol 31(2) 69-79(2019) 30299470
    Hitomi K, Tahara-Hanaoka S, Miki H, Iwata K, Shibayama S, Kubo M, Shibuya A.
    Allergin-1 on mast cells suppresses house dust mite-induced airway hyperresponsiveness in mice.
    Int Immunol 30(9) 429-434(2018) 30169732
    Nakamura T, Maeda S, Horiguchi K, Maehara T, Aritake K, Choi BI, Iwakura Y, Urade Y, Murata T.
    PGD2 deficiency exacerbates food antigen-induced mast cell hyperplasia.
    Nat Commun 6 7514(2015) 26159556
    Iwanaga K, Nakamura T, Maeda S, Aritake K, Hori M, Urade Y, Ozaki H, Murata T.
    Mast cell-derived prostaglandin D2 inhibits colitis and colitis-associated colon cancer in mice.
    Cancer Res 74(11) 3011-9(2014) 24879565
    Ohneda K, Moriguchi T, Ohmori S, Ishijima Y, Satoh H, Philipsen S, Yamamoto M.
    Transcription factor GATA1 is dispensable for mast cell differentiation in adult mice.
    Mol Cell Biol 34(10) 1812-26(2014) 24615013
    Nakahashi-Oda C, Tahara-Hanaoka S, Shoji M, Okoshi Y, Nakano-Yokomizo T, Ohkohchi N, Yasui T, Kikutani H, Honda S, Shibuya K, Nagata S, Shibuya A.
    Apoptotic cells suppress mast cell inflammatory responses via the CD300a immunoreceptor.
    J Exp Med 209(8) 1493-503(2012) 22826299
    Murata T, Aritake K, Matsumoto S, Kamauchi S, Nakagawa T, Hori M, Momotani E, Urade Y, Ozaki H.
    Prostagladin D2 is a mast cell-derived antiangiogenic factor in lung carcinoma.
    Proc Natl Acad Sci U S A 108(49) 19802-7(2011) 22106279
    Schuler P, Contassot E, Irla M, Hugues S, Preynat-Seauve O, Beermann F, Donda A, French LE, Huard B.
    Direct presentation of a melanocyte-associated antigen in peripheral lymph nodes induces cytotoxic CD8+ T cells.
    Cancer Res 68(20) 8410-8(2008) 18922914