Strain Data Sheet

RBRC01903

Strain Information

Image
BRC No.RBRC01903
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Lats2<tm1Noj>
Former Common nameTats2 heterozygous (He) mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2002. D3 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionLats2 gene knockout mice. The first coding exon was replaced with a PGK-neo cassette. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
References
Lats2 is an essential mitotic regulator required for the coordination of cell division.
Yabuta N, Okada N, Ito A, Hosomi T, Nishihara S, Sasayama Y, Fujimori A, Okuzaki D, Zhao H, Ikawa M, Okabe M, Nojima H
J. Biol. Chem., 282, 19259-19271 (2007). 17478426

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lats2
MGI:1354386		large tumor suppressor 214Lats2<tm1Noj> targeted mutation 1, Hiroshi Nojima
neo neomycin resistance gene (E. coli)14 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell morphology(MP:0000358)

  • abnormal cell nucleus morphology(MP:0003111)

  • abnormal fourth ventricle morphology(MP:0000828)

  • abnormal mitosis(MP:0004046)

  • abnormal pharyngeal arch morphology(MP:0002884)
    • more 9 phenotypes
  • abnormal spinal cord morphology(MP:0000955)

  • decreased embryo size(MP:0001698)

  • delayed muscle development(MP:0000736)

  • embryonic growth retardation(MP:0003984)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • increased cell nucleus count(MP:0003707)

  • increased spinal cord apoptosis(MP:0013182)

  • polyploidy(MP:0004025)

  • small heart(MP:0002188)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Lats2 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationCell Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 282, 19259-19271 (2007). The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorHiroshi Nojima (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data