Strain Data Sheet

RBRC01908

Strain Information

Image
BRC No.RBRC01908
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Mt1-Il5)6Ato
Former Common nameIL-5 Transgenic mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearance
Strain developmentGenerated by Kiyoshi Takatsu, Kumamoto University Medical School. The transgene was injected into the pronuclei of C3H fertilized eggs. C57BL/6 (RBRC01908) and BALB/c (RBRC05910) background (Developed by Institute of Medical Science, University of Tokyo), NZW background (RBRC01200) (Developed by Juntendo University School of Medicine).
Strain descriptionMt1-Il5 transgenic mice. These transgenic mice express mouse IL-5 under the control of the Mt1 promoter.
Colony maintenanceCarrier x Carrier (Homozygous x Homozygote) Tg mice show no obvious abnormality.
References
Transgenic mice expressing a B cell growth and differentiation factor gene (interleukin 5) develop eosinophilia and autoantibody production.
Tominaga A, Takaki S, Koyama N, Katoh S, Matsumoto R, Migita M, Hitoshi Y, Hosoya Y, Yamauchi S, Kanai Y, et a l
J. Exp. Med., 173, 429-437 (1991). 1988543

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin splice acceptorUNHBB2
HBB2 rabbit beta-globin splice donorUNHBB2
Il5
MGI:96557		interleukin 5UNIl5 metallothionein 1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • increased IgA level(MP:0002495)

  • increased IgM level(MP:0002494)

  • increased anti-double stranded DNA antibody level(MP:0004762)

  • increased circulating interleukin-5 level(MP:0008626)

  • increased eosinophil cell number(MP:0005011)
    • more 1 phenotypes
  • increased interleukin-5 secretion(MP:0008702)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse metallothionein promoter, rabbit beta-globin SD, rabbit beta-globin SA, mouse IL5 cDNA, rabbit beta-globin polyA, SV40 polyadenylation site
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 173, 429-437 (1991). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKiyoshi Takatsu (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Aihara N, Kamiie J, Yamada M, Shirota K.
    The development of mixed cryoglobulinemia in Capillaria hepatica-infected mice is associated with the capillaria antigen-induced selective proliferation of splenic B-1a cells in response to interleukin-5 stimulation.
    Am J Pathol 185(1) 172-84(2015) 25452118
    Tani Y, Isobe Y, Imoto Y, Segi-Nishida E, Sugimoto Y, Arai H, Arita M.
    Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice.
    FASEB J 28(9) 4036-43(2014) 24891522