Strain Data Sheet

RBRC01918

Strain Information

Image
BRC No.RBRC01918
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(deRFP)2Rbrc
Former Common nameOct-delta PE-mRFP#2, TgN(de RFP)2Rbrc
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kuniya Abe, RIKEN BioResrouce Center in 2004. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were crossed to C57BL/6 over N2 times.
Strain descriptionOct-4/RFP transgenic mice carry mRFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express red fluorescent protein at various stages of embryonic development.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
mRFP monomeric Red Fluorescent Protein (Discosoma sp.)UNmRFP Oct 3/4 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAOct3/4 promoter, Discosoma sp. mRFP cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 1 year after provided to the RIKEN BRC. Users should be contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorKuniya Abe (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. UCSD MTA
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data