Strain Data Sheet

RBRC01922

Strain Information

Image
BRC No.RBRC01922
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Zeb1<tm1Yhi>
Former Common nameδ EF1 [Delta]C-fin, δ EF1 [Delta]C727
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 11 times.
Strain descriptionZeb1 gene knockout mice (deltaEF1 deltaC727). Exon 6 of the Zeb1 was replaced with a neo cassette. In the genetic background of inbred mouse strains, homozygous mutant mice die after birth. While about 20-30% of homozygous mice survive to adulthood in the mixed genetic background.
Colony maintenance
References
Impairment of T cell development in deltaEF1 mutant mice.
Higashi Y, Moribe H, Takagi T, Sekido R, Kawakami K, Kikutani H, Kondoh H
J. Exp. Med., 185, 1467-1480 (1997). 9126927

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zeb1
MGI:1344313		zinc finger E-box binding homeobox 118Zeb1<tm1Yhi>
MGI:1857515		targeted mutation 1, Yujiro Higashi
  • corneal dystrophy, Fuchs endothelial, 6(MedGEN)

  • posterior polymorphous corneal dystrophy 3(MedGEN)
    • neo neomycin resistance gene (E. coli)18 SV40 early promoter and herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell differentiation(MP:0002145)

  • abnormal T cell subpopulation ratio(MP:0003944)

  • abnormal cornea endothelium morphology(MP:0005301)

  • abnormal cornea morphology(MP:0001312)

  • abnormal cornea stroma morphology(MP:0005300)
    • more 19 phenotypes
  • abnormal iridocorneal angle(MP:0004221)

  • abnormal thymus development(MP:0000704)

  • anterior iris synechia(MP:0011481)

  • ascites(MP:0005324)

  • cornea abrasion(MP:0004105)

  • cornea epithelium hypoplasia(MP:0020858)

  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased T cell number(MP:0005018)

  • decreased double-positive T cell number(MP:0005092)

  • decreased lymphocyte cell number(MP:0005016)

  • decreased splenocyte number(MP:0009339)

  • decreased thymocyte number(MP:0000715)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • lymph node hypoplasia(MP:0008101)

  • neonatal lethality, complete penetrance(MP:0011087)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • premature death(MP:0002083)

  • small thymus(MP:0000706)

  • thymus hypoplasia(MP:0001823)
    • Detailed phenotype data

    Ordering Information

    Donor DNAThymidine kinase gene promoter sequence (HSV), neomycin resistance gene coding sequence (E. coli), SV40 early gene promoter and termination sequence (SV40), mouse Zeb1 genomic DNA
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 185, 1467-1480 (1997). The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE.
    DepositorYujiro Higashi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data