Strain Data Sheet

RBRC01923

Strain Information

Image
BRC No.RBRC01923
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Zeb1<tm2Yhi>/YhiRbrc
Former Common namedelta EF1 Knockout mice, delta EF1 null (lacZ), B6;129-Zfhx1a<tm2Yhi>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University. R1 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionZeb1 gene knockout mice (deltaEF1 null lacZ). Exon 1 was replaced with a lacZ-neo cassette including termination signals. Homozygous mutant mice die shortly after birth.
Colony maintenance
References
DeltaEF1, a zinc finger and homeodomain transcription factor, is required for skeleton patterning in multiple lineages.
Takagi T, Moribe H, Kondoh H, Higashi Y
Development, 125, 21-31 (1998). 9389660

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zeb1
MGI:1344313		zinc finger E-box binding homeobox 118Zeb1<tm2Yhi>
MGI:1857515		targeted mutation 2, Yujiro Higashi
  • corneal dystrophy, Fuchs endothelial, 6(MedGEN)

  • posterior polymorphous corneal dystrophy 3(MedGEN)
    • lacZ beta-galactosidase (E. coli)18 SV40 early gene promoter and termination sequence (SV40), Thymidine kinase gene promoter sequence (HSV)
    neo neomycin resistance gene (E. coli)18 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • Meckel's cartilage hyperplasia(MP:0030373)

  • abnormal Meckel's cartilage morphology(MP:0005587)

  • abnormal basisphenoid bone morphology(MP:0000106)

  • abnormal craniofacial bone morphology(MP:0002116)

  • abnormal elbow joint morphology(MP:0013945)
    • more 52 phenotypes
  • abnormal hip joint morphology(MP:0030829)

  • abnormal jaw morphology(MP:0000454)

  • abnormal joint morphology(MP:0002932)

  • abnormal lateral semicircular canal morphology(MP:0000037)

  • abnormal mandibular angle morphology(MP:0004596)

  • abnormal nasal septum morphology(MP:0002239)

  • abnormal navicular morphology(MP:0009727)

  • abnormal patella morphology(MP:0005353)

  • abnormal phalanx morphology(MP:0005306)

  • abnormal pterygoid bone morphology(MP:0004453)

  • abnormal rib morphology(MP:0000150)

  • abnormal serous gland morphology(MP:0008052)

  • abnormal sternebra morphology(MP:0004322)

  • abnormal sternum morphology(MP:0000157)

  • abnormal tarsal bone morphology(MP:0005104)

  • abnormal vascular smooth muscle physiology(MP:0005595)

  • abnormal vascular wound healing(MP:0004883)

  • abnormal xiphoid process morphology(MP:0000159)

  • bowed radius(MP:0004374)

  • bowed tibia(MP:0004358)

  • brachyphalangia(MP:0002543)

  • circling(MP:0001394)

  • cleft secondary palate(MP:0009890)

  • curly tail(MP:0003051)

  • cyanosis(MP:0001575)

  • decreased length of long bones(MP:0004686)

  • decreased thymocyte number(MP:0000715)

  • delayed endochondral bone ossification(MP:0003419)

  • exencephaly(MP:0000914)

  • fetal growth retardation(MP:0004201)

  • fused carpal bones(MP:0008915)

  • fused metatarsal bones(MP:0004642)

  • hemorrhage(MP:0001914)

  • hydrops fetalis(MP:0002192)

  • intervertebral disk hypoplasia(MP:0004681)

  • mandible hypoplasia(MP:0000460)

  • mandibular condyloid process hypoplasia(MP:0030259)

  • mandibular coronoid process hypoplasia(MP:0030285)

  • neonatal lethality, complete penetrance(MP:0011087)

  • open neural tube(MP:0000929)

  • palate bone hypoplasia(MP:0009892)

  • patellar dislocation(MP:0030860)

  • respiratory failure(MP:0001953)

  • rib fusion(MP:0000154)

  • short limbs(MP:0000547)

  • short mandible(MP:0000088)

  • short maxilla(MP:0000097)

  • short premaxilla(MP:0000091)

  • short scapula(MP:0004340)

  • short sternum(MP:0004321)

  • syndactyly(MP:0000564)

  • thymus hypoplasia(MP:0001823)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin resistance gene coding sequence, E. coli LacZ gene coding sequence, SV40 early gene promoter and termination sequence, mouse Zeb1 genomic DNA
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 125, 21-31 (1998).The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE. The RECIPIENT should consult with the DEPOSITOR on the authorship upon publications of the results obtained from the use of the BIOLOGICAL RESOURCE. The authorship should be considered in accordance to generally accepted practices and decided through agreement between the RECIPIENT and the DEPOSITOR/DEVELOPER.
    DepositorYujiro Higashi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data