Strain Data Sheet

RBRC01925

Strain Information

Image
BRC No.RBRC01925
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129(Cg)-Zeb2<tm1.2Yhi>
Former Common nameSIP1 knockout mouse, SIP1Δex7, B6;129-Zfhx1b<tm1.2Yhi>
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University in 2002.
Strain descriptionZeb2 (Zfhx1b) knockout mice. Homozygous mutant mice are embryonic lethal. Heterozygous mutant mice are viable and fertile, but, have a tendency to exhibit growth retardation.
Colony maintenance
References
Generation of the floxed allele of the SIP1 (Smad-interacting protein 1) gene for Cre-mediated conditional knockout in the mouse.
Higashi Y, Maruhashi M, Nelles L, Van de Putte T, Verschueren K, Miyoshi T, Yoshimoto A, Kondoh H, Huylebroeck D
Genesis, 32, 82-84 (2002). 11857784

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zfhx1b
MGI:1344407		zinc finger homeobox 1b2Zfhx1b<tm1.2Yhi>
MGI:2387501		targeted mutation 1.2, Yujiro Higashi
  • Mowat-Wilson syndrome(DisGeNET, MedGEN)
    • loxP phage P1 loxP2loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cranial neural crest cell migration(MP:0012757)

  • abnormal embryo development(MP:0001672)

  • abnormal neural crest cell delamination(MP:0012746)

  • abnormal neural crest cell migration(MP:0002950)

  • abnormal neural crest cell morphology(MP:0009845)
    • more 19 phenotypes
  • abnormal neural plate morphology(MP:0005657)

  • abnormal somite development(MP:0001688)

  • abnormal somite shape(MP:0001691)

  • abnormal somite size(MP:0005222)

  • absent first pharyngeal arch(MP:0006342)

  • absent vagal neural crest cells(MP:0013011)

  • decreased body size(MP:0001265)

  • decreased somite size(MP:0011732)

  • embryonic growth arrest(MP:0001730)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • hindlimb paresis(MP:0031204)

  • incomplete embryo turning(MP:0001701)

  • nervous system phenotype(MP:0003631)

  • open neural tube(MP:0000929)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • progressive hair loss(MP:0009004)

  • vagina atresia(MP:0001144)

  • weight loss(MP:0001263)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse zfhx1b genomic DNA, phage P1 loxP
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 32, 82-84 (2002). The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE. The RECIPIENT should consult with the DEPOSITOR on the authorship upon publications of the results obtained from the use of the BIOLOGICAL RESOURCE. The authorship should be considered in accordance to generally accepted practices and decided through agreement between the RECIPIENT and the DEPOSITOR/DEVELOPER.
    DepositorYujiro Higashi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data