Strain Data Sheet

RBRC01929

Strain Information

Image
BRC No.RBRC01929
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Rab8b<tm1>
Former Common nameRab8b KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
The Rab8 GTPase regulates apical protein localization in intestinal cells.
Sato T, Mushiake S, Kato Y, Sato K, Sato M, Takeda N, Ozono K, Miki K, Kubo Y, Tsuji A, Harada R, Harada A
Nature 448, 366-369 (2007). 17597763

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site9Frt
Frt yeast FRT (flippase recombination target) site9Frt
Rab8b
MGI:2442982		RAB8B, member RAS oncogene family9Rab8b
lacZ beta-galactosidase (E. coli)9
loxP phage P1 loxP9loxP
loxP phage P1 loxP9loxP
neo neomycin resistance gene (E. coli)9

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal intestinal absorption(MP:0001666)

  • abnormal small intestine morphology(MP:0000496)

  • accumulation of giant lysosomes in kidney/renal tubule cells(MP:0005060)

  • cachexia(MP:0005150)

  • decreased small intestinal microvillus size(MP:0008141)
    • more 2 phenotypes
  • diarrhea(MP:0005036)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli neo, Phage P1 loxP, yeast FRT (flipase recombination target) sites, mouse rab8b genomic DNA, E. coli lacZ
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concerning the analysis of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorAkihiro Harada (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data