Strain Data Sheet

RBRC01949

Strain Information

Image
BRC No.RBRC01949
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4(Cg)-Tsix<tm1Sado>
Former Common nameTsix SA; Tsix splicing defect mutant mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2006. J1 ES cells were used to generate the mutant mice. Puromycin resistance was excised by crossing with cre transgenic mice.
Strain descriptionTsix gene splicing knockout mice (Tsix<SA>). The splicing acceptor site of Tsix exon 4 was replaced with a fragment containing an IRES-EGFP and a floxed puromycin resistance gene. This mutant line carries modified allele of Tsix locus to avoid the production major splicing variant. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
References
Tsix defective in splicing is competent to establish Xist silencing.
Sado T, Hoki Y, Sasaki H
Development, 133, 4925-4931 (2006). 17108001

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)XEGFP
IRES internal ribosomal entry site (EMCV)X
Tsix
MGI:1336196		X (inactive)-specific transcript, opposite strandXTsix<tm1Sado> targeted mutation 1, Takashi Sado
loxP phage P1 loxPXloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal allantois morphology(MP:0001726)

  • abnormal chorion morphology(MP:0002836)

  • abnormal developmental patterning(MP:0002084)

  • abnormal digestive system physiology(MP:0001663)

  • abnormal ectoplacental cavity morphology(MP:0011202)
    • more 9 phenotypes
  • abnormal ectoplacental cone morphology(MP:0005032)

  • abnormal extraembryonic coelom morphology(MP:0011200)

  • abnormal gastrulation(MP:0001695)

  • abnormal germ layer development(MP:0001674)

  • abnormal mesoderm development(MP:0001680)

  • abnormal visceral yolk sac morphology(MP:0001718)

  • absent egg cylinders(MP:0001694)

  • prenatal lethality, incomplete penetrance(MP:0011101)

  • small amniotic cavity(MP:0012104)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Phage P1 loxP, mouse Tsix genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 133, 4925-4931 (2006).The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
    The RECIPIENT should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorTakashi Sado (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2009

    BRC mice in Publications

    No Data