Strain Data Sheet

RBRC01950

Strain Information

Image
BRC No.RBRC01950
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Xist<tm1Sado> Tsix<tm1Enl>
Former Common nameX<dc>; Xist/Tsix double KO mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2005.
Strain descriptionXsit/Tsix double knockout mice (X<dc>). An IRES-EGFP cassette was inserted to replace part of exon 1 of Xist gene in mutated J1 ES cells lacking Tsix gene (Tsix<tm1Enl>) in which exon 2 and 3 were replaced with an IRES Beta-geo cassette. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
References
Tsix silences Xist through modification of chromatin structure.
Sado T, Hoki Y, Sasaki H
Developmental Cell, 9, 159-165 (2005). 15992549

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)XEGFP
IRES internal ribosomal entry site (EMCV)X
Tsix
MGI:1336196		X (inactive)-specific transcript, opposite strandXTsix<tm1Enl>
MGI:2679786		targeted mutation 1, En Li
Xist
MGI:98974		inactive X specific transcriptsXXist<tm1Sado> targeted mutation 1, Takashi Sado
lacZ beta-galactosidase (E. coli)X
loxP phage P1 loxPXloxP
neo neomycin resistance gene (E. coli)X

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal allantois morphology(MP:0001726)

  • abnormal amniotic cavity morphology(MP:0011199)

  • abnormal chorion morphology(MP:0002836)

  • abnormal developmental patterning(MP:0002084)

  • abnormal digestive system physiology(MP:0001663)
    • more 12 phenotypes
  • abnormal ectoplacental cavity morphology(MP:0011202)

  • abnormal ectoplacental cone morphology(MP:0005032)

  • abnormal extraembryonic coelom morphology(MP:0011200)

  • abnormal gastrulation(MP:0001695)

  • abnormal germ layer development(MP:0001674)

  • abnormal mesoderm development(MP:0001680)

  • abnormal visceral yolk sac morphology(MP:0001718)

  • absent egg cylinders(MP:0001694)

  • decreased embryo size(MP:0001698)

  • embryonic lethality between implantation and somite formation, complete penetrance(MP:0011096)

  • prenatal lethality, incomplete penetrance(MP:0011101)

  • small amniotic cavity(MP:0012104)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Xist/Tsix genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Phage P1 loxP, E. coli neo, E. coli lacZ
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell, 9, 159-165 (2005).The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
    The RECIPIENT should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorTakashi Sado (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2009

    BRC mice in Publications

    No Data