Strain Data Sheet

RBRC01952

Strain Information

Image
BRC No.RBRC01952
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Lats1<tm1Noj>
Former Common nameLats1 heterozygous (He) mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2004. D3 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionLats1 gene knockout mice (deltaN type). Exon 1 containing a translation initiation codon was replaced with a PGK-neo cassette. Homozygous mutant mice are small. Homozygous mutant females cannot nurse pups due to lacking mammae.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
References
N-terminal truncation of Lats1 causes abnormal cell growth control and chromosomal instability.
Yabuta N, Mukai S, Okamoto A, Okuzaki D, Suzuki H, Torigata K, Yoshida K, Okada N, Miura D, Ito A, Ikawa M, Okabe M, Nojima H
J. Cell Sci., 126, 508-520 (2013). 23230145

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lats1
MGI:1333883		large tumor suppressor10Lats1<tm1Noj>
MGI:3840659		targeted mutation 1, Hiroshi Nojima
neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell morphology(MP:0000358)

  • abnormal cell physiology(MP:0005621)

  • abnormal mitosis(MP:0004046)

  • abnormal mitotic spindle morphology(MP:0009760)

  • abnormal tumor morphology(MP:0003448)
    • more 5 phenotypes
  • decreased body weight(MP:0001262)

  • increased fibroblast proliferation(MP:0011703)

  • neoplasm(MP:0002006)

  • postnatal growth retardation(MP:0001732)

  • prenatal lethality, incomplete penetrance(MP:0011101)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Lats1 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 126, 508-520 (2013). The RECIPIENT agrees to use of this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorHiroshi Nojima (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data