Strain Data Sheet

RBRC01957

Strain Information

Image
BRC No.RBRC01957
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Blm<tm2Khor>
Former Common nameBlmtm2Tak
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Bloom's syndrome gene-deficient phenotype in mouse primary cells induced by a modified tetracycline-controlled trans-silencer.
Hayakawa T, Yusa K, Kouno M, Takeda J, Horie K
Gene, 369, 80-89 (2006). 16387452

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Blm
MGI:1328362		Bloom syndrome, RecQ like helicase7Blm<tm2Khor> targeted mutation 2, Kyoji Horie
  • Bloom syndrome(DisGeNET, MedGEN)
    • IRES internal ribosomal entry site (EMCV)7
    Nox4 NADPH oxidase 4 (human)7Nox4
    loxP phage P1 loxP7loxP
    neo neomycin resistance gene (E. coli)7
    tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)7tTA
    tetO tetracycline operator (E. coli)7tetO

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Tetracycline operator, E. coli Tetracycline repressor, E. coli Neomycin resistance gene, Human Kox gene,Encephalomyocarditis virus Internal ribosome entry site, Herpes simplex virus VP16 gene, Herpes simplex virus Thyidine kinase promoter, Herpes simplex virus Thymidine kinase polyA signal, P1 phage LoxP, Adenovirus Tripartite leader sequence, SV40 polyA signal, Bovine Growth hormone polyA signal
    Research applicationCre/loxP system
    Tet system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data