Strain Data Sheet

RBRC01958

Strain Information

Image
BRC No.RBRC01958
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ahnak<tm1Jtak>
Former Common nameAhnak-KO, Ahnak<tm1Tak>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Junji Takeda at Osaka University in 2001.
Strain descriptionAHNAK/Desmoyokin is a ubiquitous protein expressed in a variety of cell type, distributes mainly on the cell membranes, suggesting that this gene plays a role in cell-cell adhesion. Homozygous mutant mice are viable and fertile, and show no overt abnormalities.
Colony maintenanceBackcross to C57BL/6J
References
Ahnak/Desmoyokin is dispensable for proliferation, differentiation, and maintenance of integrity in mouse epidermis.
Kouno M, Kondoh G, Horie K, Komazawa N, Ishii N, Takahashi Y, Takeda J, Hashimoto T
J. Invest. Dermatol., 123, 700-707 (2004). 15373775

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ahnak
MGI:1316648		AHNAK nucleoprotein19Ahnak<tm1Jtak>
MGI:3053964		targeted mutation 1, Junji Takeda
loxP phage P1 loxP19loxP
loxP phage P1 loxP19loxP
neo neomycin resistance gene (E. coli)19 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Ahnak genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Invest Dermatol, 123, 700-707 (2004). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    von Boxberg Y, Soares S, Féréol S, Fodil R, Bartolami S, Taxi J, Tricaud N, Nothias F.
    Giant scaffolding protein AHNAK1 interacts with β-dystroglycan and controls motility and mechanical properties of Schwann cells.
    Glia 62(9) 1392-406(2014) 24796807