Strain Data Sheet

RBRC01967

Strain Information

Image
BRC No.RBRC01967
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Nanog<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exson 2 whcich contains the homeodomain. The mutant mice were backcross to C57BL/6J.
Strain descriptionNanog gene is a important factor of maintaining ES cell pluripotent differentiation.Homozygous mutant mice are embryonic lethal.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells.
Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S
Cell, 113, 631-642 (2003). 12787504

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRES internal ribosomal entry site (EMCV)6
Nanog
MGI:1919200		Nanog homeobox6Nanog<tm1Yam>
MGI:2664492		targeted mutation 1, Shinya Yamanaka
lacZ beta-galactosidase (E. coli)6
neo neomycin resistance gene (E. coli)6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • absent embryonic epiblast(MP:0011184)

  • absent extraembryonic ectoderm(MP:0002583)

  • disorganized extraembryonic tissue(MP:0002582)

  • embryonic lethality between implantation and somite formation, complete penetrance(MP:0011096)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Nanog genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A
    Research applicationDevelopmental Biology Research
    Specific Term and Conditions
    DepositorShinya Yamanaka (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: Japanese (Word) / English(Word)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data