Strain Data Sheet

RBRC01974

Strain Information

Image
BRC No.RBRC01974
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S6.129X1(B6)-Dsg3<tm1Stan>
Former Common nameDesmoglein 3 knockout mice
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strain129S6/SvEvTac
Appearance
Strain development
Strain description
Colony maintenance
References
Targeted disruption of the pemphigus vulgaris antigen (desmoglein 3) gene in mice causes loss of keratinocyte cell adhesion with a phenotype similar to pemphigus vulgaris.
P J Koch, M G Mahoney, H Ishikawa, L Pulkkinen, J Uitto, L Shultz, G F Murphy, D Whitaker-Menezes, J R Stanley
J. Cell Biol., 137(5):1091-102 (1997). 9166409

Antigen-independent development of Foxp3+ regulatory T cells suppressing autoantibody production in experimental pemphigus vulgaris.
Tomoaki Yokoyama, Satoshi Matsuda, Yujiro Takae, Naoko Wada, Takeji Nishikawa, Masayuki Amagai, Shigeo Koyasu
Int. Immunol., 23(6):365-73 (2011). 21525154

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dsg3
MGI:99499		desmoglein 318Dsg3<tm1Stan> targeted mutation 1, John R Stanley
  • blistering, acantholytic, of oral and laryngeal mucosa(MedGEN)
    • neo neomycin resistance gene (E. coli)18 mouse phosphoglycerate kinase promoter (PGK promoter)
    herpes simplex virus thymidine kinase gene PolyA (HSV TK gene polyA)18

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell adhesion(MP:0003566)

  • abnormal hair cycle(MP:0000427)

  • abnormal hair shaft morphology(MP:0003809)

  • acantholysis(MP:0004241)

  • alopecia(MP:0000414)
    • more 10 phenotypes
  • blistering(MP:0001208)

  • conjunctivitis(MP:0001852)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased total body fat amount(MP:0010025)

  • lethality at weaning, incomplete penetrance(MP:0011084)

  • mouth mucosal ulcer(MP:0003747)

  • postnatal growth retardation(MP:0001732)

  • skin lesions(MP:0001212)

  • weight loss(MP:0001263)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPGK promoter (mouse phosphoglycerate kinase gene promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptII) gene of the transposon Tn5 in Escherichia coli K12), herpes simplex virus thymidine kinase gene PolyA (HSV TK gene Poly A)
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol., 23(6):365-73 (2011).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data