Strain Data Sheet

RBRC01984

Strain Information

Image
BRC No.RBRC01984
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6C3F1-Tg(CAG-EGFP)C15-FM042Osb
Former Common name
H-2 Haplotype
ES Cell line
Background strainSlc:B6C3F1
Appearance
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.
Strain descriptionThese transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a CAG promoter. Transgene integration site: 15D.
Colony maintenanceCarrier x Carrier [or Crossing to Slc:B6C3F1]
References
FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y, Okabe M
Genomics. 80(6):564-574 (2002). 12504848

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)15 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACX-EGFP (Jellyfish GFP cDNA, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA))
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics. 80(6):564-574 (2002).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data