Strain Data Sheet

RBRC02090

Strain Information

Image
BRC No.RBRC02090
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Pcdha<tm(AdAB)> Olfr17<tm1Mom/tm7Mom>
Former Common nameP2-LacZ/P2-GFP/AdAB
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. The mutant mice were generated by crossing with CNR AdAB (RBRC01687) (Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University), P2-lacZ and P2-GFP (Peter Monbaerts, The Rockefeller University) mice.
Strain descriptionCNR (Pcdha) AdAB mutant (CR1, CR2 and CR3a were deleted) and P2 (Olfr17) gene ires-taulacZ and ires-tauGFP knock-in triple mutant mice.
Colony maintenancePcdha: Homozygote x Homozygote; lacZ: Heterozygote x Heterozygote; GFP: Heterozygote x Heterozygote
References
Visualizing an olfactory sensory map.
Mombaerts P, Wang F, Dulac C, Chao S K, Nemes A, Mendelsohn M, Edmondson J, Axel R
Cell, 87, 675-686 (1996). 8929536

The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse.
Hasegawa S, Hamada S, Kumode Y, Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T
Mol Cell Neurosci, 38, 66-79 (2008). 18353676

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pcdha
MGI:3719085		protocadherin alpha cluster18Pcdha<tm(AdAB)> targeted mutation
loxP phage P1 loxP18loxP
GFP Green Fluorescent Protein (Aequorea victoria)7
IRES internal ribosomal entry site (EMCV)7
IRES internal ribosomal entry site (EMCV)7
Olfr17
MGI:109148		olfactory receptor 177Olfr17<tm1Mom>
MGI:2179510		targeted mutation 1, Peter Mombaerts
Olfr17
MGI:109148		olfactory receptor 177Olfr17<tm7Mom>
MGI:3692295		targeted mutation 7, Peter Mombaerts
lacZ beta-galactosidase (E. coli)7
loxP phage P1 loxP7loxP
loxP phage P1 loxP7loxP
tau bovine tau protein cDNA7tau
tau bovine tau protein cDNA7tau

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal olfactory neuron innervation pattern (MP:0012014)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP, E. coli LacZ, Encephalomyocarditis virus ires (internal ribosone entry site), jellyfish green fluorescent protein (GFP) cDNA, bovine tau cDNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior consent on use of it from Professor Peter Monbaerts, The Rockefeller University. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate the treatment of such invention. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT, which wishes to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese), Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data