Strain Data Sheet

RBRC02095

Strain Information

Image
BRC No.RBRC02095
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Th-EGFP)21-31Koba
Former Common nameB6.Cg-Tg(TH-GFP)21-31
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain descriptionThe TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson’s disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
Matsushita N, Okada H, Yasoshima Y, Takahashi K, Kiuchi K, Kobayashi K
J. Neurochem., 82, 295-304 (2002). 12124430

Health Report

Examination Date / Room / Rack2024/11/18Room:4-ARack:KSentinel mouse program
2024/08/19Room:4-ARack:KSentinel mouse program
2024/05/20Room:4-ARack:KSentinel mouse program
2024/02/19Room:4-ARack:KSentinel mouse program
2023/11/20Room:4-ARack:KSentinel mouse program
2023/08/21Room:4-ARack:KSentinel mouse program
2023/05/22Room:4-ARack:KSentinel mouse program
2023/02/20Room:4-ARack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN rat tyrosine hydroxylase (TH) promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
  • Detailed phenotype data

    Ordering Information

    Donor DNArat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jelly fish green fluorescent protein (GFP) gene, rabbit beta-globin poly A, SV40 early gene poly A
    Research applicationFluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 82, 295-304 (2002).
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month May 2008

    Construct map
    Transgene sequence

    BRC mice in Publications

    Heo JY, Nam MH, Yoon HH, Kim J, Hwang YJ, Won W, Woo DH, Lee JA, Park HJ, Jo S, Lee MJ, Kim S, Shim JE, Jang DP, Kim KI, Huh SH, Jeong JY, Kowall NW, Lee J, Im H, Park JH, Jang BK, Park KD, Lee HJ, Shin H, Cho IJ, Hwang EM, Kim Y, Kim HY, Oh SJ, Lee SE, Paek SH, Yoon JH, Jin BK, Kweon GR, Shim I, Hwang O, Ryu H, Jeon SR, Lee CJ.
    Aberrant Tonic Inhibition of Dopaminergic Neuronal Activity Causes Motor Symptoms in Animal Models of Parkinson's Disease.
    Curr Biol 30(2) 276-291.e9(2020) 31928877
    Kosaka T, Pignatelli A, Kosaka K.
    Heterogeneity of tyrosine hydroxylase expressing neurons in the main olfactory bulb of the mouse.
    Neurosci Res 157 15-33(2020) 31629793
    Kiyokage E, Kobayashi K, Toida K.
    Spatial distribution of synapses on tyrosine hydroxylase-expressing juxtaglomerular cells in the mouse olfactory glomerulus.
    J Comp Neurol 525(5) 1059-1074(2017) 27864931
    Masi A, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G.
    Differential contribution of Ih to the integration of excitatory synaptic inputs in substantia nigra pars compacta and ventral tegmental area dopaminergic neurons.
    Eur J Neurosci 42(9) 2699-706(2015) 26354486
    Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K.
    Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb.
    J Comp Neurol 523(2) 262-80(2015) 25234191
    Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J.
    Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking.
    Nat Neurosci 18(8) 1084-93(2015) 26147533
    Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K.
    Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb.
    J Neurosci 31(32) 11587-96(2011) 21832189