Strain Information | |
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Image | |
BRC No. | RBRC02095 |
Type | Transgene Congenic![]() |
Species | Mus musculus |
Strain name | B6.B6D2-Tg(Th-EGFP)21-31Koba |
Former Common name | B6.Cg-Tg(TH-GFP)21-31 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162. |
Strain description | The TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson’s disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development. Matsushita N, Okada H, Yasoshima Y, Takahashi K, Kiuchi K, Kobayashi K J. Neurochem., 82, 295-304 (2002). 12124430 |
Health Report | |
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Examination Date / Room / Rack | 2024/11/18Room:4-ARack:KSentinel mouse program 2024/08/19Room:4-ARack:KSentinel mouse program 2024/05/20Room:4-ARack:KSentinel mouse program 2024/02/19Room:4-ARack:KSentinel mouse program 2023/11/20Room:4-ARack:KSentinel mouse program 2023/08/21Room:4-ARack:KSentinel mouse program 2023/05/22Room:4-ARack:KSentinel mouse program 2023/02/20Room:4-ARack:KSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
GFP | Green Fluorescent Protein (Aequorea victoria) | UN | rat tyrosine hydroxylase (TH) promoter |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | rat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jelly fish green fluorescent protein (GFP) gene, rabbit beta-globin poly A, SV40 early gene poly A |
Research application | Fluorescent Proteins/lacZ System Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 82, 295-304 (2002). |
Depositor | Kazuto Kobayashi (Fukushima Medical University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2008 Construct map Transgene sequence |
BRC mice in Publications |
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Heo JY, Nam MH, Yoon HH, Kim J, Hwang YJ, Won W, Woo DH, Lee JA, Park HJ, Jo S, Lee MJ, Kim S, Shim JE, Jang DP, Kim KI, Huh SH, Jeong JY, Kowall NW, Lee J, Im H, Park JH, Jang BK, Park KD, Lee HJ, Shin H, Cho IJ, Hwang EM, Kim Y, Kim HY, Oh SJ, Lee SE, Paek SH, Yoon JH, Jin BK, Kweon GR, Shim I, Hwang O, Ryu H, Jeon SR, Lee CJ. Aberrant Tonic Inhibition of Dopaminergic Neuronal Activity Causes Motor Symptoms in Animal Models of Parkinson's Disease. Curr Biol 30(2) 276-291.e9(2020) 31928877 |
Kosaka T, Pignatelli A, Kosaka K. Heterogeneity of tyrosine hydroxylase expressing neurons in the main olfactory bulb of the mouse. Neurosci Res 157 15-33(2020) 31629793 |
Kiyokage E, Kobayashi K, Toida K. Spatial distribution of synapses on tyrosine hydroxylase-expressing juxtaglomerular cells in the mouse olfactory glomerulus. J Comp Neurol 525(5) 1059-1074(2017) 27864931 |
Masi A, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G. Differential contribution of Ih to the integration of excitatory synaptic inputs in substantia nigra pars compacta and ventral tegmental area dopaminergic neurons. Eur J Neurosci 42(9) 2699-706(2015) 26354486 |
Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K. Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb. J Comp Neurol 523(2) 262-80(2015) 25234191 |
Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J. Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking. Nat Neurosci 18(8) 1084-93(2015) 26147533 |
Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K. Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb. J Neurosci 31(32) 11587-96(2011) 21832189 |