Strain Data Sheet

RBRC02098

Strain Information

Image
BRC No.RBRC02098
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Plxna2<tm1Hfu>/HfuRbrc
Former Common namePlexin-A2 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrSlc
Appearance
Strain development
Strain description
Colony maintenance
References
Interactions between plexin-A2, plexin-A4, and semaphorin 6A control lamina-restricted projection of hippocampal mossy fibers.
Suto F, Tsuboi M, Kamiya H, Mizuno H, Kiyama Y, Komai S, Shimizu M, Sanbo M, Yagi T, Hiromi Y, Chedotal A, Mitchell K J, Manabe T, Fujisawa H
Neuron, 53, 535-547 (2007) 17296555

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRES internal ribosomal entry site (EMCV)1
Plxna2
MGI:107684		plexin A21Plxna2<tm1Hfu>
MGI:3793763		targeted mutation 1, Hajime Fujisawa
Tak E. coli Tet repressor DNA binding protein (TetR), HSV VP16 transactivating domain cDNA1Tak
loxP phage P1 loxP1loxP
loxP phage P1 loxP1loxP
loxP phage P1 loxP1loxP
neo neomycin resistance gene (E. coli)1 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal amacrine cell morphology(MP:0005240)

  • abnormal dendrite morphology(MP:0008143)

  • abnormal hippocampal mossy fiber morphology(MP:0002761)

  • abnormal lymphatic vessel morphology(MP:0001879)

  • abnormal neuron physiology(MP:0004811)
    • more 6 phenotypes
  • abnormal neuronal migration(MP:0006009)

  • abnormal nursing(MP:0001382)

  • abnormal retina inner plexiform layer morphology(MP:0003734)

  • ectopic cerebellar granule cells(MP:0002762)

  • reduced male fertility(MP:0001922)

  • vision/eye phenotype(MP:0005391)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Encephalomyocarditis virus IRES, Tak (E. coli Tet repressor DNA binding protein (TetR), HSV VP16 transactivating domain cDNA), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA, mouse plexin-A2 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Suto et al., Neuron, 53(4):535-547 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr.
    Fumikazu Suto, National Institute of Neuroscience, National Center of Neurology and Psychiatry, E-mail: fsuto@ncnp.go.jp) to transfer the BIOLOGICAL RESOURCE.
    DepositorHajime Fujisawa (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data