Strain Data Sheet

RBRC02142

Strain Information

Image
BRC No.RBRC02142
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S2-Pdcd1<tm1Hon>/HonRbrc
Former Common nameC57BL/6-PD-1KO, C57BL/6-Pdcd1-/- mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6
Appearanceblack [(a/a B/B C/C)]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University in 1998. The knockout construct was electoroporated into D3 ES cells derived from 129S2/SvPas. The mutant mice were backcrossed to C57BL/6 over 11 generations.
Strain descriptionPD1 knockout mice. EcoRV site of exon 3 to PvuII site of exon 5 was replaced with noemycin selection cassette. PD1 (Pdcd1, programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, plays a role in the negative control of proliferation, differentiation and class switching of B cells. PD1 deficiency has been shown to develop different forms of autoimmune diseases on different backgrounds of mice. Homozygous PD1 mutant mice of C57BL/6 background show moderate but consistent splenomegaly, and spontaneously develop characteristic lupus-like proliferative arthritis and golmerulonephritis with the age.
Colony maintenanceHomozygote x Homozygote.
References
Immunological studies on PD-1 deficient mice: implication of PD-1 as a negative regulator for B cell responses.
Nishimura H, Minato N, Nakano T, Honjo T
Int. Immunol.,10,1563-1572 (1998). 9796923

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Nishimura H, Nose M, Hiai H, Minato N, Honjo T
Immunity, 11, 141-151 (1999). 10485649

Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice.
Nishimura H, Okazaki T, Tanaka Y, Nakatani K, Hara M, Matsumori A, Sasayama S, Mizoguchi A, Hiai H, Minato N, Honjo T
Science, 291, 319-322 (2001). 11209085

Health Report

Examination Date / Room / Rack2024/12/09Room:3-3Rack:ASentinel mouse program
2024/09/09Room:3-3Rack:ASentinel mouse program
2024/06/10Room:3-3Rack:ASentinel mouse program
2024/03/11Room:3-3Rack:ASentinel mouse program
2023/12/11Room:3-3Rack:ASentinel mouse program
2023/09/11Room:3-3Rack:ASentinel mouse program
2023/06/13Room:3-3Rack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pdcd1
MGI:104879		programmed cell death 11Pdcd1<tm1Hon>
MGI:2386184		targeted mutation 1, Tasuku Honjo
  • multiple sclerosis, susceptibility to(MedGEN)

  • systemic lupus erythematosus, susceptibility to, 2(MedGEN)
    • neo neomycin resistance gene (E. coli)1 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell activation(MP:0001828)

  • abnormal cardiac muscle contractility(MP:0002972)

  • abnormal cardiac output(MP:0001627)

  • abnormal cardiovascular system physiology(MP:0001544)

  • abnormal heart morphology(MP:0000266)
    • more 29 phenotypes
  • abnormal immunoglobulin level(MP:0002490)

  • abnormal leukopoiesis(MP:0005460)

  • abnormal myelopoiesis(MP:0001601)

  • abnormal response to transplant(MP:0005671)

  • arthritis(MP:0002993)

  • cardiac fibrosis(MP:0003141)

  • decreased susceptibility to experimental autoimmune encephalomyelitis(MP:0004800)

  • dilated heart ventricle(MP:0008022)

  • enlarged heart(MP:0000274)

  • enlarged liver(MP:0000599)

  • enlarged spleen(MP:0000691)

  • exophthalmos(MP:0002750)

  • glomerulonephritis(MP:0002743)

  • hepatic necrosis(MP:0001654)

  • increased B cell number(MP:0005014)

  • increased B cell proliferation(MP:0005154)

  • increased B-1 B cell number(MP:0004977)

  • increased IgA level(MP:0002495)

  • increased IgG2b level(MP:0008501)

  • increased IgG3 level(MP:0008502)

  • increased IgM level(MP:0002494)

  • increased autoantibody level(MP:0003725)

  • increased inflammatory response(MP:0001846)

  • increased interferon-gamma secretion(MP:0008566)

  • increased lymphocyte cell number(MP:0005013)

  • pancreas necrosis(MP:0009148)

  • premature death(MP:0002083)

  • pulmonary necrosis(MP:0010857)

  • thin ventricular wall(MP:0000280)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Jul 2012

    BRC mice in Publications

    Akama Y, Park EJ, Satoh-Takayama N, Ito A, Kawamoto E, Gaowa A, Matsuo E, Oikawa S, Saito M, Inoue S, Akimoto T, Suzuki K, Shimaoka M.
    Roles of programmed death-1 and muscle innate lymphoid cell-derived interleukin 13 in sepsis-induced intensive care unit-acquired weakness.
    J Cachexia Sarcopenia Muscle (2024) 39016179
    Gao Y, Song B, Aoki S, Ito K.
    Conjugation of human serum albumin and flucloxacillin provokes specific immune response in HLA-B*57:01 transgenic mice.
    Immunol Lett 249 5-11(2022) 35963284
    Miyajima M, Zhang B, Sugiura Y, Sonomura K, Guerrini MM, Tsutsui Y, Maruya M, Vogelzang A, Chamoto K, Honda K, Hikida T, Ito S, Qin H, Sanuki R, Suzuki K, Furukawa T, Ishihama Y, Matsuda F, Suematsu M, Honjo T, Fagarasan S.
    Metabolic shift induced by systemic activation of T cells in PD-1-deficient mice perturbs brain monoamines and emotional behavior.
    Nat Immunol 18(12) 1342-1352(2017) 29058703
    Horne-Debets JM, Faleiro R, Karunarathne DS, Liu XQ, Lineburg KE, Poh CM, Grotenbreg GM, Hill GR, MacDonald KP, Good MF, Renia L, Ahmed R, Sharpe AH, Wykes MN.
    PD-1 dependent exhaustion of CD8+ T cells drives chronic malaria.
    Cell Rep 5(5) 1204-13(2013) 24316071
    Yokosuka T, Takamatsu M, Kobayashi-Imanishi W, Hashimoto-Tane A, Azuma M, Saito T.
    Programmed cell death 1 forms negative costimulatory microclusters that directly inhibit T cell receptor signaling by recruiting phosphatase SHP2.
    J Exp Med 209(6) 1201-17(2012) 22641383
    Kawamoto S, Tran TH, Maruya M, Suzuki K, Doi Y, Tsutsui Y, Kato LM, Fagarasan S.
    The inhibitory receptor PD-1 regulates IgA selection and bacterial composition in the gut.
    Science 336(6080) 485-9(2012) 22539724
    Mumprecht S, Schürch C, Schwaller J, Solenthaler M, Ochsenbein AF.
    Programmed death 1 signaling on chronic myeloid leukemia-specific T cells results in T-cell exhaustion and disease progression.
    Blood 114(8) 1528-36(2009) 19420358