Strain Data Sheet

RBRC02182

Strain Information

Image
BRC No.RBRC02182
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Prm1-Cre)01Osb
Former Common nameprotamine-cre
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masato Ikawa at Osaka University in 1997.
Strain descriptionThis transgenic mouse expresses the cre-recombinase under the control of the protamine 1 gene promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have floxed genes.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL6/CrSlc)
References
Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Matsumura H, Hasuwa H, Inoue N, Ikawa M, Okabe M
Biochem. Biophys. Res. Commun., 321, 275-279 (2004) 15358172

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre protamine 1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • azoospermia(MP:0005159)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse protaminel promoter, Phage P1 cre recombinase DNA, rabbit glogin polyA signal
    Research applicationCre/loxP system
    Reproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophs. Res. Commun., 321, 275-279 (2004).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Miao J, Chen W, Wang P, Zhang X, Wang L, Wang S, Wang Y.
    MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice.
    Int J Mol Sci 22(24) (2021) 34948301