Strain Information | |
---|---|
Image | ![]() |
BRC No. | RBRC02192 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.129-Mapk14<tm1.2Otsu> |
Former Common name | p38α-flox mouse |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Kinya Otsu, Osaka University Graduate School of Medicine in 2000. The targeting construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J. |
Strain description | Mitogen-activated protein (MAP) kinase cascades are highly conserved signal transduction pathways involved in a variety of physiological events. The MAP kinase family consists of extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal protein kinase (JNK), and p38 MAP kinase. p38 alpha, one of the p38 MAP kinase subfamily, is widely expressed and has an important function in cytokine production and the response to many types of stress. Because p38alpha-deficient mice (BRC00361 C57BL/6-TgH(p38)/118) showed embryonic lethality at midgestation, this strain is useful for the research of p38 MAP kinase pathway in various tissues using Cre-loxP system. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile. |
References | p38alpha mitogen-activated protein kinase plays a critical role in cardiomyocyte survival but not in cardiac hypertrophic growth in response to pressure overload. Nishida K, Yamaguchi O, Hirotani S, Hikoso S, Higuchi Y, Watanabe T, Takeda T, Osuka S, Morita T, Kondoh G, Uno Y, Kashiwase K, Taniike M, Nakai A, Matsumura Y, Miyazaki J, Sudo T, Hongo K, Kusakari Y, Kurihara S, Chien K R, Takeda J, Hori M, Otsu K Mol. Cell. Biol., 24, 10611-10620 (2004). 15572667 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Mapk14 MGI:1346865 | mitogen-activated protein kinase 14 | 17 | Mapk14<tm1.2Otsu> MGI:3525576 | targeted mutation 1.2, Kinya Otsu | |||
loxP | phage P1 loxP | 17 | loxP | ||||
loxP | phage P1 loxP | 17 | loxP |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | Phage P1 loxP sites, mouse p38 alpha genomic DNA |
Research application | Cell Biology Research Cre/loxP system General Purpose |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 24, 10611-10620 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Kinya Otsu (Osaka University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jul 2007 |
BRC mice in Publications |
---|
Luo Q, Schnöder L, Hao W, Litzenburger K, Decker Y, Tomic I, Menger MD, Liu Y, Fassbender K. p38α-MAPK-deficient myeloid cells ameliorate symptoms and pathology of APP-transgenic Alzheimer's disease mice. Aging Cell 21(8) e13679(2022) 35909315 |
Schnöder L, Tomic I, Schwindt L, Helm D, Rettel M, Schulz-Schaeffer W, Krause E, Rettig J, Fassbender K, Liu Y. P38α-MAPK phosphorylates Snapin and reduces Snapin-mediated BACE1 transportation in APP-transgenic mice. FASEB J 35(7) e21691(2021) 34118085 |
Schnöder L, Gasparoni G, Nordström K, Schottek A, Tomic I, Christmann A, Schäfer KH, Menger MD, Walter J, Fassbender K, Liu Y. Neuronal deficiency of p38α-MAPK ameliorates symptoms and pathology of APP or Tau-transgenic Alzheimer's mouse models. FASEB J 34(7) 9628-9649(2020) 32475008 |
Kase Y, Otsu K, Shimazaki T, Okano H. Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling. Stem Cell Reports 12(6) 1313-1328(2019) 31080114 |
Schnöder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y. Deficiency of Neuronal p38α MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1. J Biol Chem 291(5) 2067-79(2016) 26663083 |
Krementsov DN, Noubade R, Dragon JA, Otsu K, Rincon M, Teuscher C. Sex-specific control of central nervous system autoimmunity by p38 mitogen-activated protein kinase signaling in myeloid cells. Ann Neurol 75(1) 50-66(2014) 24027119 |
Hutchison MR. Mice with a conditional deletion of the neurotrophin receptor TrkB are dwarfed, and are similar to mice with a MAPK14 deletion. PLoS One 8(6) e66206(2013) 23776632 |
Liu X, Ma B, Malik AB, Tang H, Yang T, Sun B, Wang G, Minshall RD, Li Y, Zhao Y, Ye RD, Xu J. Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases. Nat Immunol 13(5) 457-64(2012) 22447027 |