Strain Data Sheet

RBRC02192

Strain Information

Image
BRC No.RBRC02192
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mapk14<tm1.2Otsu>
Former Common namep38α-flox mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kinya Otsu, Osaka University Graduate School of Medicine in 2000. The targeting construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionMitogen-activated protein (MAP) kinase cascades are highly conserved signal transduction pathways involved in a variety of physiological events. The MAP kinase family consists of extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal protein kinase (JNK), and p38 MAP kinase. p38 alpha, one of the p38 MAP kinase subfamily, is widely expressed and has an important function in cytokine production and the response to many types of stress. Because p38alpha-deficient mice (BRC00361 C57BL/6-TgH(p38)/118) showed embryonic lethality at midgestation, this strain is useful for the research of p38 MAP kinase pathway in various tissues using Cre-loxP system.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
References
p38alpha mitogen-activated protein kinase plays a critical role in cardiomyocyte survival but not in cardiac hypertrophic growth in response to pressure overload.
Nishida K, Yamaguchi O, Hirotani S, Hikoso S, Higuchi Y, Watanabe T, Takeda T, Osuka S, Morita T, Kondoh G, Uno Y, Kashiwase K, Taniike M, Nakai A, Matsumura Y, Miyazaki J, Sudo T, Hongo K, Kusakari Y, Kurihara S, Chien K R, Takeda J, Hori M, Otsu K
Mol. Cell. Biol., 24, 10611-10620 (2004). 15572667

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mapk14
MGI:1346865
mitogen-activated protein kinase 1417Mapk14<tm1.2Otsu>
MGI:3525576
targeted mutation 1.2, Kinya Otsu
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse p38 alpha genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    General Purpose
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 24, 10611-10620 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKinya Otsu (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Jul 2007

    BRC mice in Publications

    Luo Q, Schnöder L, Hao W, Litzenburger K, Decker Y, Tomic I, Menger MD, Liu Y, Fassbender K.
    p38α-MAPK-deficient myeloid cells ameliorate symptoms and pathology of APP-transgenic Alzheimer's disease mice.
    Aging Cell 21(8) e13679(2022) 35909315
    Schnöder L, Tomic I, Schwindt L, Helm D, Rettel M, Schulz-Schaeffer W, Krause E, Rettig J, Fassbender K, Liu Y.
    P38α-MAPK phosphorylates Snapin and reduces Snapin-mediated BACE1 transportation in APP-transgenic mice.
    FASEB J 35(7) e21691(2021) 34118085
    Schnöder L, Gasparoni G, Nordström K, Schottek A, Tomic I, Christmann A, Schäfer KH, Menger MD, Walter J, Fassbender K, Liu Y.
    Neuronal deficiency of p38α-MAPK ameliorates symptoms and pathology of APP or Tau-transgenic Alzheimer's mouse models.
    FASEB J 34(7) 9628-9649(2020) 32475008
    Kase Y, Otsu K, Shimazaki T, Okano H.
    Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling.
    Stem Cell Reports 12(6) 1313-1328(2019) 31080114
    Schnöder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y.
    Deficiency of Neuronal p38α MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1.
    J Biol Chem 291(5) 2067-79(2016) 26663083
    Krementsov DN, Noubade R, Dragon JA, Otsu K, Rincon M, Teuscher C.
    Sex-specific control of central nervous system autoimmunity by p38 mitogen-activated protein kinase signaling in myeloid cells.
    Ann Neurol 75(1) 50-66(2014) 24027119
    Hutchison MR.
    Mice with a conditional deletion of the neurotrophin receptor TrkB are dwarfed, and are similar to mice with a MAPK14 deletion.
    PLoS One 8(6) e66206(2013) 23776632
    Liu X, Ma B, Malik AB, Tang H, Yang T, Sun B, Wang G, Minshall RD, Li Y, Zhao Y, Ye RD, Xu J.
    Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases.
    Nat Immunol 13(5) 457-64(2012) 22447027