Strain Data Sheet

RBRC02227

Strain Information

Image
BRC No.RBRC02227
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Ripply2<tm1.1Ysa>/YsaRbrc
Former Common namedelta-neo-Ripply2-KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
The negative regulation of Mesp2 by mouse Ripply2 is required to establish the rostro-caudal patterning within a somite.
Morimoto M, Sasaki N, Oginuma M, Kiso M, Igarashi K, Aizaki K, Kanno J, Saga Y
Development, 134, 1561-1569 (2007). 17360776

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ripply2
MGI:2685968		ripply transcriptional repressor 29Ripply2<tm1.1Ysa> targeted mutation 1.1, Yumiko Saga
  • spondylocostal dysostosis 6, autosomal recessive(MedGEN)
    • loxP phage P1 loxP9loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal myotome morphology(MP:0003939)

  • abnormal vertebrae morphology(MP:0000137)

  • abnormal vertebral pedicle morphology(MP:0004604)

  • absent proximal rib(MP:0000152)

  • absent vertebral pedicles(MP:0000140)
    • more 4 phenotypes
  • decreased body length(MP:0001258)

  • failure of somite differentiation(MP:0001690)

  • neonatal lethality, complete penetrance(MP:0011087)

  • short tail(MP:0000592)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Ripply2 genomic DNA
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 134, 1561-1569 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    McInerney-Leo AM, Sparrow DB, Harris JE, Gardiner BB, Marshall MS, O'Reilly VC, Shi H, Brown MA, Leo PJ, Zankl A, Dunwoodie SL, Duncan EL.
    Compound heterozygous mutations in RIPPLY2 associated with vertebral segmentation defects.
    Hum Mol Genet 24(5) 1234-42(2015) 25343988