Strain Data Sheet

RBRC02228

Strain Information

Image
BRC No.RBRC02228
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(TOPGAL)/YsaRbrc
Former Common nameTOP-GAL
H-2 Haplotype
ES Cell line
Background strainICR
Appearancealbino [c/c]
Strain developmentDeveloped by Yumiko Saga, National Institute of Genetics in 2005. The transgene was injected into the pronuclei of (C57BL/6 x C3H)F2 fertilized eggs. ICR mixed background.
Strain descriptionTransgenic mice for visualizing Wnt signal pathway. Wnt proteins belong to a family of secreted and lipid-modified glycoprotein that can activate intracellular signaling pathways, such as the Wnt/planar cell polarity, the Wnt/calcium and the Wnt beta-catenin or so-called Wnt canonical signaling pathways. The TOP-GAL transgenic mice are a reporter line which can visualize the Wnt canonical pathway by beta-galactosidase activity. The transgenic mice were generated with ins-TOPGAL that accurately drive beta-galactosidase under TCF/beta-catenin. These transgenic mice will provide you unique opportunity to investigate the function of Wnt canonical pathway in vivo.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]. Tg mice are viable and fertile.
References
GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
Moriyama A, Kii I, Sunabori T, Kurihara S, Takayama I, Shimazaki M, Tanabe H, Oginuma M, Fukayama M, Matsuzaki Y, Saga Y, Kudo A
Genesis, 45, 90-100 (2007) 17245775

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
lacZ beta-galactosidase (E. coli)UN TOPFLUSH promoter
nls SV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNATCF binding site from TOPFLUSH vector (Upstate) , HSV TK promoter, E. coli LacZ, SV40-late-poly A, chicken β-globin HS4 insulater, Simian virus 40 Large T antigen nuclear localization signal (nls)
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    General Purpose
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 45, 90-100 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Aug 2009

    BRC mice in Publications

    Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, Kominami R.
    Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after γ-irradiation through Wnt/β-catenin pathway.
    Carcinogenesis 36(6) 622-31(2015) 25827435
    Lu TL, Chen CM.
    Differential requirements for β-catenin in murine prostate cancer originating from basal versus luminal cells.
    J Pathol 236(3) 290-301(2015) 25712462
    Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y.
    β-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia.
    Sci Rep 4 6959(2014) 25376241
    Garbe DS, Ring RH.
    Investigating tonic Wnt signaling throughout the adult CNS and in the hippocampal neurogenic niche of BatGal and ins-TopGal mice.
    Cell Mol Neurobiol 32(7) 1159-74(2012) 22491991