Strain Data Sheet

RBRC02256

Strain Information

Image
BRC No.RBRC02256
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Grin2a<tm1Nak>
Former Common nameNR2A KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionNR2A (Grin2a) gene knockout mice. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunit.
Kadotani H, Hirano T, Masugi M, Nakamura K, Nakao K, Katsuki M, Nakanishi S
J. Neurosci., 16, 7859-7867 (1996). 8987814

Health Report

Examination Date / Room / Rack2024/11/25Room:4-BRack:ESentinel mouse program
2024/08/26Room:4-BRack:ESentinel mouse program
2024/05/27Room:4-BRack:ESentinel mouse program
2024/02/26Room:4-BRack:ESentinel mouse program
2023/11/27Room:4-BRack:ESentinel mouse program
2023/08/30Room:4-BRack:EAdditional tests
2023/08/28Room:4-BRack:ESentinel mouse program
2023/05/29Room:4-BRack:ESentinel mouse program
2023/03/22Room:4-BRack:EAdditional tests

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grin2a
MGI:95820		glutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2a<tm1Nak>
MGI:1928506		targeted mutation 1, Shigetada Nakanishi
  • Landau-Kleffner syndrome(MedGEN)
    • neo neomycin resistance gene (E. coli)16 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal excitatory postsynaptic currents(MP:0002910)

  • abnormal miniature excitatory postsynaptic currents(MP:0004753)

  • behavior/neurological phenotype(MP:0005386)

  • decreased vertical activity(MP:0002757)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse NMDAR2A genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 16, 7859-7867 (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data