Strain Data Sheet

RBRC02298

Strain Information

Image
BRC No.RBRC02298
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Il3<tm1Glli>
Former Common nameIL-3 knock-out mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cAnNTac
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Glenn Dranoff, Dana-Farber Cancer Institute, Harvard Medical School in 1998. Mutant mice were backcrossed to C57BL/6 for 9 gnerations, and then to BALB/cAnNTac for 3 generations. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine.
Strain descriptionInterluekin-3 is a growth factor of hematopoieic progenitors that yields multipotential blast cells, mast cells, basophils,neutrophils, macrophages, eosinophils, erythrocytes, megakaryocytes, and dendritic cells. IL-3 null mutant mice show unimpaired hematopoiesis, but contact hypersensitivity reactions are compromised.
Colony maintenanceBackcross to BALB/c (Heterozygote x BALB/cAnNTac)
References
Involvement of interleukin-3 in delayed-type hypersensitivity.
Mach N, Lantz C S, Galli S J, Reznikoff G, Mihm M, Small C, Granstein R, Beissert S, Sadelain M, Mulligan R C, Dranoff G
Blood, 91, 778-783 (1998). 9446636

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Il3
MGI:96552		interleukin 311Il3<tm1Glli>
MGI:2446538		targeted mutation 1, Stephen J Galli
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)
poly A mouse PGK polyA11poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal mast cell differentiation(MP:0005462)

  • abnormal pancreatic islet morphology(MP:0005215)

  • decreased susceptibility to type IV hypersensitivity reaction(MP:0005616)

  • increased susceptibility to parasitic infection(MP:0005027)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Il3 genomic DNA, mouse Phosphoglycerate kinase-1(PGK) promoter, E. coli neo, mouse PGK polyA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Glenn Dranoff, MD, Harvard Medical School, Dana-Farber Cancer Institute E-mail: glenn_dranoff@dfci.harvard.edu).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Bénard A, Mittelstädt A, Klösch B, Glanz K, Müller J, Schoen J, Nüse B, Brunner M, Naschberger E, Stürzl M, Mattner J, Muñoz LE, Sohn K, Grützmann R, Weber GF.
    IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils.
    Cell Rep 42(6) 112637(2023) 37300834
    Julia Ko¨lle, Theodor Zimmermann, Alexander Kiefer, Ralf J. Rieker, Paraskevi Xepapadaki, Sebastian Zundler, Nikolaos G. Papadopoulos, Susetta Finotto
    Targeted deletion of Interleukin-3 results in asthma exacerbations
    iScience 104440(2022) 35707726
    Balam S, Schiechl-Brachner G, Buchtler S, Halbritter D, Schmidbauer K, Talke Y, Neumayer S, Salewski JN, Winter F, Karasuyama H, Yamanishi Y, Renner K, Geissler EK, Mack M.
    IL-3 Triggers Chronic Rejection of Cardiac Allografts by Activation of Infiltrating Basophils.
    J Immunol 202(12) 3514-3523(2019) 31068389
    Weber GF, Chousterman BG, He S, Fenn AM, Nairz M, Anzai A, Brenner T, Uhle F, Iwamoto Y, Robbins CS, Noiret L, Maier SL, Zönnchen T, Rahbari NN, Schölch S, Klotzsche-von Ameln A, Chavakis T, Weitz J, Hofer S, Weigand MA, Nahrendorf M, Weissleder R, Swirski FK.
    Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis.
    Science 347(6227) 1260-5(2015) 25766237
    Motomura Y, Morita H, Moro K, Nakae S, Artis D, Endo TA, Kuroki Y, Ohara O, Koyasu S, Kubo M.
    Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation.
    Immunity 40(5) 758-71(2014) 24837103
    Leyva-Castillo JM, Hener P, Michea P, Karasuyama H, Chan S, Soumelis V, Li M.
    Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade.
    Nat Commun 4 2847(2013) 24284909