Strain Data Sheet

RBRC02302

Strain Information

Image
BRC No.RBRC02302
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Lyzs<tm1(cre)Ifo>
Former Common nameLysM-Cre knock-in mice (C57BL/6), B6.129P2-Lyzs<tm1(cre)Ifo>
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6NTac
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Technical University of Munich, Irmgard Foerster. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303).
Strain descriptionLyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References
Conditional gene targeting in macrophages and granulocytes using LysMcre mice.
Clausen B E, Burkhardt C, Reith W, Renkawitz R, Forster I
Transgenic Res., 8, 265-277 (1999) 10621974

Health Report

Examination Date / Room / Rack2024/12/09Room:3-5Rack:ASentinel mouse program
2024/09/09Room:3-5Rack:ASentinel mouse program
2024/06/10Room:3-5Rack:ASentinel mouse program
2024/03/11Room:3-5Rack:ASentinel mouse program
2023/12/11Room:3-5Rack:ASentinel mouse program
2023/09/11Room:3-5Rack:ASentinel mouse program
2023/06/13Room:3-5Rack:ASentinel mouse program
2023/03/13Room:3-5Rack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Lyz2
MGI:96897
lysozyme 210Lyz2<tm1(cre)Ifo>
MGI:1934631
targeted mutation 1, Irmgard Foerster
  • familial visceral amyloidosis(MedGEN)
  • cre Phage P1 Cre recombinase10cre
    nls SV40 large T antigen nuclear localization signal (NLS)10nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • enlarged spleen(MP:0000691)
  • Detailed phenotype data

    Ordering Information

    Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), Phage P1 cre, yeast FRT (flipase recombination target) site, mouse lysozyme M genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de.
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Suzuki T, Taketomi Y, Yanagida K, Yoshida-Hashidate T, Nagase T, Murakami M, Shimizu T, Shindou H.
    Re-evaluation of the canonical PAF pathway in cutaneous anaphylaxis.
    Biochim Biophys Acta Mol Cell Biol Lipids 1870(1) 159563(2024) 39332666
    Kunanopparat A, Leelahavanichkul A, Visitchanakun P, Kueanjinda P, Phuengmaung P, Sae-Khow K, Boonmee A, Benjaskulluecha S, Palaga T, Hirankarn N.
    The Regulatory Roles of Ezh2 in Response to Lipopolysaccharide (LPS) in Macrophages and Mice with Conditional Ezh2 Deletion with LysM-Cre System.
    Int J Mol Sci 24(6) (2023) 36982437
    Saisorn W, Phuengmaung P, Issara-Amphorn J, Makjaroen J, Visitchanakun P, Sae-Khow K, Boonmee A, Benjaskulluecha S, Nita-Lazar A, Palaga T, Leelahavanichkul A.
    Less Severe Lipopolysaccharide-Induced Inflammation in Conditional mgmt-Deleted Mice with LysM-Cre System: The Loss of DNA Repair in Macrophages.
    Int J Mol Sci 24(12) (2023) 37373287
    Phuengmaung P, Khiewkamrop P, Makjaroen J, Issara-Amphorn J, Boonmee A, Benjaskulluecha S, Ritprajak P, Nita-Lazar A, Palaga T, Hirankarn N, Leelahavanichkul A.
    Less Severe Sepsis in Cecal Ligation and Puncture Models with and without Lipopolysaccharide in Mice with Conditional Ezh2-Deleted Macrophages (LysM-Cre System).
    Int J Mol Sci 24(10) (2023) 37239864
    Mitsui K, Hishiyama S, Jain A, Kotoda Y, Abe M, Matsukawa T, Kotoda M.
    Role of macrophage autophagy in postoperative pain and inflammation in mice.
    J Neuroinflammation 20(1) 102(2023) 37131209
    Sato H, Taketomi Y, Miki Y, Murase R, Yamamoto K, Murakami M.
    Secreted Phospholipase PLA2G2D Contributes to Metabolic Health by Mobilizing ω3 Polyunsaturated Fatty Acids in WAT.
    Cell Rep 31(5) 107579(2020) 32375030
    Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T, Deguchi Y, Tamai I.
    Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation during Fever.
    J Neurosci 38(24) 5584-5595(2018) 29899035
    Akbari M, Honma K, Kimura D, Miyakoda M, Kimura K, Matsuyama T, Yui K.
    IRF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major.
    J Immunol 192(5) 2271-9(2014) 24489086