Strain Information | |
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Image | ![]() |
BRC No. | RBRC02302 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.129P2-Lyzs<tm1(cre)Ifo> |
Former Common name | LysM-Cre knock-in mice (C57BL/6), B6.129P2-Lyzs<tm1(cre)Ifo> |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6NTac |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Technical University of Munich, Irmgard Foerster. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303). |
Strain description | Lyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NTac] |
References | Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Clausen B E, Burkhardt C, Reith W, Renkawitz R, Forster I Transgenic Res., 8, 265-277 (1999) 10621974 |
Health Report | |
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Examination Date / Room / Rack | 2024/12/09Room:3-5Rack:ASentinel mouse program 2024/09/09Room:3-5Rack:ASentinel mouse program 2024/06/10Room:3-5Rack:ASentinel mouse program 2024/03/11Room:3-5Rack:ASentinel mouse program 2023/12/11Room:3-5Rack:ASentinel mouse program 2023/09/11Room:3-5Rack:ASentinel mouse program 2023/06/13Room:3-5Rack:ASentinel mouse program 2023/03/13Room:3-5Rack:ASentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 10 | Frt | ||||
Lyz2 MGI:96897 | lysozyme 2 | 10 | Lyz2<tm1(cre)Ifo> MGI:1934631 | targeted mutation 1, Irmgard Foerster | |||
cre | Phage P1 Cre recombinase | 10 | cre | ||||
nls | SV40 large T antigen nuclear localization signal (NLS) | 10 | nls |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), Phage P1 cre, yeast FRT (flipase recombination target) site, mouse lysozyme M genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system Immunology and Inflammation Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de. |
Depositor | Shigeo Koyasu (Keio University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Suzuki T, Taketomi Y, Yanagida K, Yoshida-Hashidate T, Nagase T, Murakami M, Shimizu T, Shindou H. Re-evaluation of the canonical PAF pathway in cutaneous anaphylaxis. Biochim Biophys Acta Mol Cell Biol Lipids 1870(1) 159563(2024) 39332666 |
Kunanopparat A, Leelahavanichkul A, Visitchanakun P, Kueanjinda P, Phuengmaung P, Sae-Khow K, Boonmee A, Benjaskulluecha S, Palaga T, Hirankarn N. The Regulatory Roles of Ezh2 in Response to Lipopolysaccharide (LPS) in Macrophages and Mice with Conditional Ezh2 Deletion with LysM-Cre System. Int J Mol Sci 24(6) (2023) 36982437 |
Saisorn W, Phuengmaung P, Issara-Amphorn J, Makjaroen J, Visitchanakun P, Sae-Khow K, Boonmee A, Benjaskulluecha S, Nita-Lazar A, Palaga T, Leelahavanichkul A. Less Severe Lipopolysaccharide-Induced Inflammation in Conditional mgmt-Deleted Mice with LysM-Cre System: The Loss of DNA Repair in Macrophages. Int J Mol Sci 24(12) (2023) 37373287 |
Phuengmaung P, Khiewkamrop P, Makjaroen J, Issara-Amphorn J, Boonmee A, Benjaskulluecha S, Ritprajak P, Nita-Lazar A, Palaga T, Hirankarn N, Leelahavanichkul A. Less Severe Sepsis in Cecal Ligation and Puncture Models with and without Lipopolysaccharide in Mice with Conditional Ezh2-Deleted Macrophages (LysM-Cre System). Int J Mol Sci 24(10) (2023) 37239864 |
Mitsui K, Hishiyama S, Jain A, Kotoda Y, Abe M, Matsukawa T, Kotoda M. Role of macrophage autophagy in postoperative pain and inflammation in mice. J Neuroinflammation 20(1) 102(2023) 37131209 |
Sato H, Taketomi Y, Miki Y, Murase R, Yamamoto K, Murakami M. Secreted Phospholipase PLA2G2D Contributes to Metabolic Health by Mobilizing ω3 Polyunsaturated Fatty Acids in WAT. Cell Rep 31(5) 107579(2020) 32375030 |
Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T, Deguchi Y, Tamai I. Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation during Fever. J Neurosci 38(24) 5584-5595(2018) 29899035 |
Akbari M, Honma K, Kimura D, Miyakoda M, Kimura K, Matsuyama T, Yui K. IRF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major. J Immunol 192(5) 2271-9(2014) 24489086 |