Strain Data Sheet

RBRC02308

Strain Information

Image
BRC No.RBRC02308
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cxcl14<tm1>
Former Common nameB6BRAKwt/flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Ryu-Ichiro Hata, Kanagawa Dental College in 2008.
Strain descriptionC57BL/6, Cxcl14(BRAK) gene exon2 was substituted with floxp neo gene
Colony maintenanceSibling Mating (Heterozygote x Heterozygote, Wild-type)
References
BRAK/CXCL14 expression suppresses tumor growth in vivo in human oral carcinoma cells.
Ozawa S, Kato Y, Komori R, Maehata Y, Kubota E, Hata R
Biochem. BIophys. Res. Commun., 348, 406-412 (2006). 16884687

Inflamm. Regen., 30, 536-541 (2010).

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcl14
MGI:1888514		C-X-C motif chemokine ligand 1413Cxcl14<tm1Tha> targeted mutation 1, Takahiko Hara
loxP phage P1 loxP13loxP
loxP phage P1 loxP13loxP
neo neomycin resistance gene (E. coli)13 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • perinatal lethality, incomplete penetrance(MP:0011090)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Cxcl14 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The first paper making use of the donated mouse must be collaboration with the DEPOSITOR.
    DepositorRyu-Ichiro Hata (Kanagawa Dental College)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Iannone AF, Akgül G, Zhang R, Wacks S, Hussein N, Macias CG, Donatelle A, Bauriedel JMJ, Wright C, Abramov D, Johnson MA, Govek EE, Burré J, Milner TA, De Marco García NV.
    The chemokine Cxcl14 regulates interneuron differentiation in layer I of the somatosensory cortex.
    Cell Rep 43(8) 114531(2024) 39058591