Strain Data Sheet

RBRC02316

Strain Information

Image
BRC No.RBRC02316
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ndn<tm1Ky>
Former Common nameNecdin KO, C57BL/6
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuaki Yoshikawa, Osaka University in 1999.
Strain description
Colony maintenance
References
Disruption of the paternal necdin gene diminishes TrkA signaling for sensory neuron survival.
Kuwako K, Hosokawa A, Nishimura I, Uetsuki T, Yamada M, Nada S, Okada M, Yoshikawa K
J. Neurosci., 25, 7090-7099 (2005). 16049186

Necdin promotes GABAergic neuron differentiation in cooperation with Dlx homeodomain proteins.
Kuwajima T, Nishimura I, Yoshikawa K
J. Neurosci., 26, 5383-5392 (2006) 16707790

Necdin downregulates CDC2 expression to attenuate neuronal apoptosis.
Kurita M, Kuwajima T, Nishimura I, Yoshikawa K
J. Neurosci., 26, 12003-12013 (2006) 17108174

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ndn
MGI:97290		necdin, MAGE family member7Ndn<tm1Ky>
MGI:3586988		targeted mutation 1, Kazuaki Yoshikawa
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)
poly A polyA7poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal GABAergic neuron morphology(MP:0003245)

  • abnormal dentate gyrus morphology(MP:0000812)

  • abnormal dorsal root ganglion morphology(MP:0000961)

  • abnormal hippocampus development(MP:0000808)

  • abnormal neuron apoptosis(MP:0003202)
    • more 9 phenotypes
  • abnormal spatial reference memory(MP:0008414)

  • decreased body temperature(MP:0005534)

  • decreased circulating thyroid-stimulating hormone level(MP:0005119)

  • decreased circulating thyroxine level(MP:0005478)

  • decreased circulating triiodothyronine level(MP:0005479)

  • decreased glutamic acid decarboxylase level(MP:0002868)

  • increased thermal nociceptive threshold(MP:0001973)

  • maternal imprinting(MP:0003122)

  • respiratory failure(MP:0001953)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA signal, mouse Ndn genomic DNA
    Research applicationDevelopmental Biology Research
    Mouse Models for Human Disease
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT needs to contact the DEPOSITOR for a reciprocal exchange of information on the use of the RESOURCE. The RECIPIENT may be requested to collaborate with the DEPOSITOR depending on the research contents.
    DepositorKazuaki Yoshikawa (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data