Strain Data Sheet

RBRC02317

Strain Information

Image
BRC No.RBRC02317
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Pde10a2<tm1(tTA)Yok>
Former Common namePDE10A2-tTA
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Yokoi, Columbia University in 2002.
Strain descriptionPde10a2 tTA knock-in mice. A tTA gene was knocked into PDE10A2 gene locus, and the PDE10A2 gene is knocked out. The homozygotes and heterozygotes can be used as PDE10A2-knockout mice, and as a tool for the specific expression in striatal projection neurons, respectively.
Colony maintenance
References
Striatal medium spiny neurons terminate in a distinct region in the lateral hypothalamic area and do not directly innervate orexin/hypocretin- or melanin-concentrating hormone-containing neurons.
Sano H, Yokoi M
J. Neurosci., 27, 6948-6955 (2007) 17596443

Inducible expression of retrograde transynaptic genetic tracer in mice.
Sano H, Nagai Y, Yokoi M
Genetics, 45, 123-128 (2007) 17299748

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pde10a
MGI:1345143		phosphodiesterase 10A17Pde10a<tm1(tTA)Yok> targeted mutation 1, Yokoi Mineto
  • obsolete dyskinesia, limb and orofacial, infantile-onset(MedGEN)

  • striatal degeneration, autosomal dominant 2(MedGEN)
    • loxP phage P1 loxP17loxP
    tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)17tTA

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal social investigation(MP:0001360)
    • Detailed phenotype data

    Ordering Information

    Donor DNAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), Phage P1 loxP site, mouse Pde10a2 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 6948-6955 (2007). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
    DepositorYokoi Mineto (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Suzuki E, Momiyama T.
    M1 muscarinic acetylcholine receptor-mediated inhibition of GABA release from striatal medium spiny neurons onto cholinergic interneurons.
    Eur J Neurosci 53(3) 796-813(2021) 33270289