Strain Data Sheet

RBRC02330

Strain Information

Image
BRC No.RBRC02330
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameNOD.Cg-Fcer1g<tm1Rav> Fcgr2b<tm1Ttk>
Former Common nameNODFcγRnull
H-2 Haplotype
ES Cell line
Background strain
Appearancealbino [A/A c/c]
Strain developmentDeveloped by Drs. Y. Inoue and T. Takai, Tohoku University in 2004.
Strain descriptionFcR common gamma-chain (Fc-Rgamma) deficient mice. NOD background.
Colony maintenanceSibling Mating (Homozygote x Homozygote)This strain shows less severe insulitis, whereas after the onset of diabetes, homozygous and wild-type mice shows cimparable nsulitis indices.
References
FcR gamma chain deletion results in pleiotrophic effector cell defects.
Takai T, Li M, Sylvestre D, Clynes R, Ravetch J V
Cell, 76(3):519-29 (1994). 8313472

Augmented humoral and anaphylactic responses in Fc gamma RII-deficient mice.
Takai T, Ono M, Hikida M, Ohmori H, Ravetch J V
Nature, 379, 346-349 (1996). 8552190

Activating Fc gamma receptors participate in the development of autoimmune diabetes in NOD mice.
Inoue Y, Kaifu T, Sugahara-Tobinai A, Nakamura A, Miyazaki J, Takai T
J. Immunol., 179, 764-774 (2007). 17617565

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fcer1g
MGI:95496
Fc receptor, IgE, high affinity I, gamma polypeptide1Fcer1g<tm1Rav>
MGI:1857165
targeted mutation 1, Jeffrey V Ravetch
Fcgr2b
MGI:95499
Fc receptor, IgG, low affinity IIb1Fcgr2b<tm1Ttk>
MGI:1857166
targeted mutation 1, Toshiyuki Takai
neo neomycin resistance gene (E. coli)1 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
neo neomycin resistance gene (E. coli)1 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal NK cell physiology(MP:0010766)

  • abnormal dendritic cell antigen presentation(MP:0002455)

  • abnormal hemoglobin(MP:0001588)

  • abnormal hypersensitivity reaction(MP:0002148)

  • abnormal immune cell physiology(MP:0001819)
  • more 45 phenotypes
  • abnormal immunoglobulin level(MP:0002490)

  • abnormal mast cell physiology(MP:0002423)

  • abnormal platelet activation(MP:0006298)

  • abnormal seizure response to electrical stimulation(MP:0001650)

  • anemia(MP:0001577)

  • decreased body weight(MP:0001262)

  • decreased interleukin-1 beta secretion(MP:0008658)

  • decreased interleukin-10 secretion(MP:0008661)

  • decreased interleukin-12b secretion(MP:0008670)

  • decreased interleukin-23 secretion(MP:0008691)

  • decreased interleukin-6 secretion(MP:0008706)

  • decreased myocardial infarct size(MP:0003038)

  • decreased platelet aggregation(MP:0009549)

  • decreased platelet calcium level(MP:0004867)

  • decreased susceptibility to experimental autoimmune encephalomyelitis(MP:0004800)

  • decreased susceptibility to parasitic infection(MP:0005026)

  • decreased susceptibility to type I hypersensitivity reaction(MP:0005597)

  • decreased tumor necrosis factor secretion(MP:0008561)

  • dehydration(MP:0001429)

  • edema(MP:0001785)

  • enlarged lymph nodes(MP:0000702)

  • enlarged spleen(MP:0000691)

  • glomerulonephritis(MP:0002743)

  • hematoma(MP:0008817)

  • hunched posture(MP:0001505)

  • immune system phenotype(MP:0005387)

  • impaired macrophage phagocytosis(MP:0001798)

  • increased IgE level(MP:0002497)

  • increased IgG2a level(MP:0008500)

  • increased immunoglobulin level(MP:0002461)

  • increased inflammatory response(MP:0001846)

  • increased susceptibility to induced arthritis(MP:0003724)

  • increased susceptibility to parasitic infection(MP:0005027)

  • increased susceptibility to type I hypersensitivity reaction(MP:0005596)

  • increased urine protein level(MP:0002962)

  • kidney inflammation(MP:0001859)

  • liver inflammation(MP:0001860)

  • lung inflammation(MP:0001861)

  • maximal tonic hindlimb extension seizures(MP:0002194)

  • mortality/aging(MP:0010768)

  • pancreas inflammation(MP:0001869)

  • salivary gland inflammation(MP:0001870)

  • skeleton phenotype(MP:0005390)

  • tonic-clonic seizures(MP:0003997)

  • vascular inflammation(MP:0001864)
  • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Fcer1g genomic DNA, mouse Fcgr2b genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 179, 764-774 (2007).
    DepositorToshiyuki Takai (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Umemoto S, Nakahashi-Ouchida R, Yuki Y, Kurokawa S, Machita T, Uchida Y, Mori H, Yamanoue T, Shibata T, Sawada SI, Ishige K, Hirano T, Fujihashi K, Akiyoshi K, Kurashima Y, Tokuhara D, Ernst PB, Suzuki M, Kiyono H.
    Cationic-nanogel nasal vaccine containing the ectodomain of RSV-small hydrophobic protein induces protective immunity in rodents.
    NPJ Vaccines 8(1) 106(2023) 37488116
    Kayes T, Fang Y, Yu S, Downey E, Wang S, Braley-Mullen H.
    Agonistic anti-CD40 induces thyrocyte proliferation and promotes thyroid autoimmunity by increasing CD40 expression on thyroid epithelial cells.
    J Immunol 190(8) 3928-38(2013) 23509363