Strain Data Sheet

RBRC02340

Strain Information

Image
BRC No.RBRC02340
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(bitetO-GFP)BYoko
Former Common namebitetO-GFP
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Columbia University in 2002. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionThis transgenic mice express a bitetO-GFP contained the bidirectional tetracycline operator (tetO) promoter driving EGFP and a rabbit beta-globin intron/polyA signal in one direction, and in the opposite direction, driving an artifical intron, an artificial fusion gene consisting of a placental alkaline phosphatase fragment, tetanus toxin C fragment, tyrosine phosphatase delta fragment, and glutamate receptor 2 fragment (RT8) and an SV40 polyadenylation signal. This strain can be used to make an inducible expression of EGFP crossing with the genetically-engineered mice that have a tetracycline-controlled transactivator (tTA).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]Tg/+ hemizygous mice are viable and fertile.
References
Striatal medium spiny neurons terminate in a distinct region in the lateral hypothalamic area and do not directly innervate orexin/hypocretin- or melanin-concentrating hormone-containing neurons.
Sano H, Yokoi M
J. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN E. coli tetO (tetracycline operator)
Gria2 glutamate receptor, ionotropic, AMPA2 (alpha 2)UNGria2
HBB2 rabbit beta-globin intron/polyA signalUNHBB2
PLAP human placental alkaline phosphataseUNPLAP
Ptprd
MGI:97812		protein tyrosine phosphatase, receptor type, DUNPtprd
TTC non-toxic c fragment (Clostridium tetani)UNTTC
poly A SV40 polyA signalUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli tetO, CMV minimal promoter, Aequoria Victoria GFP cDNA, Rabbit beta-Globin intron/polyA signal, Human placental alkaline phosphatase, Clostridium tetani Non-toxic c fragment, Mouse tyrosine phosphatase δ(fragment), Mouse glutamate receptor 2 (fragment), SV40 polyA signal
    Research applicationFluorescent Proteins/lacZ System
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 6948-6955 (2007). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
    DepositorYokoi Mineto (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Yamada M, Suzuki Y, Nagasaki SC, Okuno H, Imayoshi I.
    Light Control of the Tet Gene Expression System in Mammalian Cells.
    Cell Rep 25(2) 487-500.e6(2018) 30304687