Strain Data Sheet

RBRC02344

Strain Information

Image
BRC No.RBRC02344
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameFVB.129P2-Nf2<tm2Gth>
Former Common nameNf2flox2 (FVB)
H-2 Haplotype
ES Cell line
Background strainFVB/NJcl
Appearancealbino [A/A B/B c/c D/D P/P]
Strain developmentDeveloped by Drs. Marco Giovannini and Gilles Thomas in INSERM at 1988. Strain of Origin: 129P2/OlaHsd-Hprt1<b-m3> via HM-1 ES cell line. FVB background.
Strain descriptionNf2, neurofibromatosis 2 is a tumor suppressor gene encoding a putative cytoskeletal associated protein. This conditional knockout mice have a loxP-flanked Nf2 allele. Conditional Nf2 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceSibling Mating (Homozygote x Homozygote, Heterozygote)Homozygous mutant mice are viable and fertile.
References
Conditional biallelic Nf2 mutation in the mouse promotes manifestations of human neurofibromatosis type 2.
Giovannini M, Robanus-Maandag E, van der Valk M, Niwa-Kawakita M, Abramowski V, Goutebroze L, Woodruff J M, Berns A, Thomas G
Genes Dev., 14, 1617-1630 (2000). 10887156

Health Report

Examination Date / Room / Rack2024/11/25Room:4-BRack:KSentinel mouse program
2024/08/26Room:4-BRack:KSentinel mouse program
2024/05/27Room:4-BRack:KSentinel mouse program
2024/02/26Room:4-BRack:KSentinel mouse program
2023/11/27Room:4-BRack:KSentinel mouse program
2023/08/28Room:4-BRack:KSentinel mouse program
2023/05/29Room:4-BRack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nf2
MGI:97307		neurofibromin 211Nf2<tm2Gth>
MGI:1926955		targeted mutation 2, Gilles Thomas
  • familial meningioma(MedGEN)

  • meningioma(DisGeNET)

  • neurofibromatosis type 2(DisGeNET, MedGEN)
    • more 1 Diseases
  • schwannomatosis 1(MedGEN)
    • loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Schwann cell morphology(MP:0001106)

  • abnormal auditory brainstem response waveform shape(MP:0011966)

  • abnormal bile duct epithelium morphology(MP:0013611)

  • abnormal osteoblast morphology(MP:0004986)

  • abnormal renal tubule morphology(MP:0002703)
    • more 37 phenotypes
  • abnormal thyroid gland morphology(MP:0000681)

  • abnormal tooth development(MP:0000116)

  • cataract(MP:0001304)

  • decreased survivor rate(MP:0008770)

  • delayed tooth eruption(MP:0003053)

  • enlarged dorsal root ganglion(MP:0008490)

  • enlarged liver(MP:0000599)

  • failure of tooth eruption(MP:0000121)

  • head bobbing(MP:0001410)

  • head tossing(MP:0005307)

  • impaired swimming(MP:0001522)

  • increased Schwann cell number(MP:0010742)

  • increased Schwannoma incidence(MP:0010310)

  • increased extremity angiosarcoma incidence(MP:0002049)

  • increased fibroadenoma incidence(MP:0010364)

  • increased hamartoma incidence(MP:0010306)

  • increased lung adenocarcinoma incidence(MP:0002027)

  • increased meningioma incidence(MP:0010311)

  • increased mesothelioma incidence(MP:0010309)

  • increased neurofibrosarcoma incidence(MP:0002030)

  • increased odontoma incidence(MP:0010353)

  • increased odontosarcoma incidence(MP:0010354)

  • increased osteoma incidence(MP:0010313)

  • increased osteosarcoma incidence(MP:0003789)

  • increased renal carcinoma incidence(MP:0010384)

  • increased sarcoma incidence(MP:0002032)

  • increased susceptibility to otitis media(MP:0001850)

  • increased tumor incidence(MP:0002020)

  • increased uterus leiomyoma incidence(MP:0003570)

  • infertility(MP:0001924)

  • neoplasm(MP:0002006)

  • nervous system phenotype(MP:0003631)

  • osseous metaplasia(MP:0010347)

  • postnatal lethality, complete penetrance(MP:0011085)

  • premature death(MP:0002083)

  • sensorineural hearing loss(MP:0004740)

  • trunk curl(MP:0001512)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Nf2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 14, 1617-1630 (2000). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT shall keep DEPOSITOR apprised of any results obtained from the BIOLOGICAL RESOURCE in the course of the research, as soon as they are available. Moreover, if the research results in a potential invention or a substance that may have potential commercial value (hereinafter referred as to the "Invention") RECIPIENT will promptly disclose this Invention to DEPOSITOR with samples thereof for DEPOSITOR's internal use.
    RECIPIENT and DEPOSITOR will consult together and agree about the possibility of filing one or more patent application to Invention conceived by one or more employees of Recipients that directly arise out of the research. The Parties will negotiate in good faith the conditions of such patent application according to each party's inventiveness contribution in the Invention.
    In case of such Invention, patentable or not, reduced in practice or developed in the course of the research that could not have been obtained, reduced to practice or developed but from using the Material and/or Confidential Information, RECIPIENT and DEPOSITOR shall confer with one another as regards licensing and commercialization of such Invention to negotiate in good faith the terms of an institutional agreement as regards said matters.
    DepositorMarco Giovannini (INSERM)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    Strain AvailabilityCryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Moon KH, Kim HT, Lee D, Rao MB, Levine EM, Lim DS, Kim JW.
    Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development.
    Dev Cell 44(1) 13-28.e3(2018) 29249622
    Ahmad I, Fernando A, Gurgel R, Jason Clark J, Xu L, Hansen MR.
    Merlin status regulates p75(NTR) expression and apoptotic signaling in Schwann cells following nerve injury.
    Neurobiol Dis 82 114-122(2015) 26057084