Strain Data Sheet

RBRC02368

Strain Information

Image
BRC No.RBRC02368
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nr1i2<tm1(NR1I2)>
Former Common name
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Jun Kanno, National Institute of Health Sciences in 2006. TT2 ES cells were used. The mutant mice were crossed to C57BL/6. Cre- recombined allele (RBRC02367) type mice were generated from Targeted allele type (RBRC02368) mice by crossing with CAG-Cre mice to removed a floxed neo cassette.
Strain descriptionhSXRki mice in which the ligand binding domain (LBD) of human SXR was knocked-into the mouse SXR gene. These umanized mouse model is a useful tool for monitoring the systemic response induced by human-selective SXR ligans. Cre- recombined allele (RBRC02367), Targeted allele type (RBRC02368).
Colony maintenance
References
Development of humanized steroid and xenobiotic receptor mouse by homologous knock-in of the human steroid and xenobiotic receptor ligand binding domain sequence.
Igarashi K, Kitajima S, Aisaki K, Tanemura K, Taquahashi Y, Moriyama N, Ikeno E, Matsuda N, Saga Y, Blumberg B, Kanno J
J. Toxicol. Sci., 37, 373-380 (2012). 22467028

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)16GH
NR1I2 Nuclear receptor subfamily 1, group I, member 2 (human)16NR1I2
Nr1i2
MGI:1337040		nuclear receptor subfamily 1, group I, member 216Nr1i2<tm1(NR1I2)> targeted mutation 1 (NR1I2)
loxP phage P1 loxP16loxP
loxP phage P1 loxP16loxP
neo neomycin resistance gene (E. coli)16 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal metabolism(MP:0005266)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman NR1I2 cDNA, Bovine growth hormone poly A, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nr1i2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Toxicol. Sci., 37, 373-380 (2012). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorJun Kanno (National Institute of Health Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data