Strain Data Sheet

RBRC02369

Strain Information

Image
BRC No.RBRC02369
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Rsph1<tm1Htan>
Former Common name
H-2 Haplotype
ES Cell lineW9.5 [129S1/Sv-Oca2<+> Tyr<+> Kitl<+>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Meichroacidin containing the membrane occupation and recognition nexus motif is essential for spermatozoa morphogenesis.
Tokuhiro K, Hirose M, Miyagawa Y, Tsujimura A, Irie S, Isotani A, Okabe M, Toyama Y, Ito C, Toshimori K, Takeda K, Oshio S, Tainaka H, Tsuchida J, Okuyama A, Nishimune Y, Tanaka H
J. Biol. Chem., 283(27):19039-19048(2008). 18453535

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rsph1
MGI:1194909
radial spoke head 1 homolog (Chlamydomonas)17Rsph1<tm1Htan> targeted mutation 1, Hiromitsu Tanaka
  • primary ciliary dyskinesia 24(MedGEN)
  • neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal male reproductive system physiology(MP:0003698)

  • abnormal reproductive system morphology(MP:0002160)

  • abnormal sperm physiology(MP:0004543)

  • abnormal spermatid morphology(MP:0006380)

  • decreased body weight(MP:0001262)
  • more 5 phenotypes
  • growth/size/body region phenotype(MP:0005378)

  • homeostasis/metabolism phenotype(MP:0005376)

  • male infertility(MP:0001925)

  • oligozoospermia(MP:0002687)

  • reproductive system phenotype(MP:0005389)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse Rsph1(meichroacidin) genomic DNA
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHiromitsu Tanaka (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Yin W, Livraghi-Butrico A, Sears PR, Rogers TD, Burns KA, Grubb BR, Ostrowski LE.
    Mice with a Deletion of Rsph1 Exhibit a Low Level of Mucociliary Clearance and Develop a Primary Ciliary Dyskinesia Phenotype.
    Am J Respir Cell Mol Biol 61(3) 312-321(2019) 30896965