Strain Information | |
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Image | ![]() |
BRC No. | RBRC02370 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.129-Grn<tm1Mni> |
Former Common name | Progranulin knock-out mouse, B6;129-Grn<tm1> |
H-2 Haplotype | |
ES Cell line | E14.1 [129P2/OlaHsd] |
Background strain | C57BL/6 |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Masugi Nishihara, Veterinary Medical Science, The University of Tokyo and Yoichiro Iwakura, Institute of Medical Science, The University of Tokyo in 2001. Targeting construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. |
Strain description | Grn, granulin knockout mice. A 4.7 kb fragment encoding exon 2-13 of Grn gene was replaced with a PGK-neo-bpA cassette. Homozygous mutant mice show a decrease in ejaculation incidence, enhanced aggressiveness and an elevated level of anxiety in male. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJmsSlc]. |
References | Alteration of behavioural phenotype in mice by targeted disruption of the progranulin gene. Kayasuga Y, Chiba S, Suzuki M, Kikusui T, Matsuwaki T, Yamanouchi K, Kotaki H, Horai R, Iwakura Y, Nishihara M Behav. Brain Res., 185, 110-118 (2007). 17764761 |
Health Report | |
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Examination Date / Room / Rack | 2024/06/10Room:3-3Rack:DSentinel mouse program 2024/03/11Room:3-3Rack:DSentinel mouse program 2023/12/11Room:3-3Rack:DSentinel mouse program 2023/09/11Room:3-3Rack:DSentinel mouse program 2023/06/13Room:3-3Rack:DSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
GH | Growth hormone polyA (Bovine) | 11 | GH | ||||
Grn MGI:95832 | granulin | 11 | Grn<tm1Mni> MGI:3762618 | targeted mutation1, Masugi Nishihara | |||
neo | neomycin resistance gene (E. coli) | 11 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Bovine growth hormone poly A signal, mouse Grn genomic DNA |
Research application | Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Behav. Brain. Res., 185, 110-118 (2007). |
Depositor | Masugi Nishihara (The University of Tokyo) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Adachi E, Murakoshi M, Shibata T, Shimozawa K, Sakuma H, Kishida C, Gohda T, Suzuki Y. Progranulin deficiency attenuates tubulointerstitial injury in a mouse unilateral ureteral obstruction model. Exp Anim (2024) 38369347 |
Tsuji S, Kudo U, Takahashi K, Nakamura S, Shimazawa M. The role of progranulin in macrophages of a glioblastoma model. J Neurooncol (2024) 39141257 |
Takahashi H, Bhagwagar S, Nies SH, Ye H, Han X, Chiasseu MT, Wang G, Mackenzie IR, Strittmatter SM. Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase. Nat Commun 15(1) 1434(2024) 38365772 |
Sasaki T, Kuse Y, Nakamura S, Shimazawa M. Progranulin-deficient macrophages cause cardiotoxicity under hypoxic conditions. Biochem Biophys Res Commun 691 149341(2023) 38039836 |
Sasaki T, Kuse Y, Nakamura S, Shimazawa M, Hara H. Progranulin deficiency exacerbates cardiac remodeling after myocardial infarction. FASEB Bioadv 5(10) 395-411(2023) 37810172 |
Nies SH, Takahashi H, Herber CS, Huttner A, Chase A, Strittmatter SM. Spreading of Alzheimer tau seeds is enhanced by aging and template matching with limited impact of amyloid-β. J Biol Chem 297(4) 101159(2021) 34480901 |
Murakoshi M, Gohda T, Adachi E, Ichikawa S, Hagiwara S, Suzuki Y. Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice. Sci Rep 11(1) 1194(2021) 33441916 |
Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M. Microglia increases the proliferation of retinal precursor cells during postnatal development. Mol Vis 24 536-545(2018) 30090016 |
Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H. Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina. Sci Rep 6 23811(2016) 27030285 |
Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, Yoshikawa H. Progranulin plays crucial roles in preserving bone mass by inhibiting TNF-α-induced osteoclastogenesis and promoting osteoblastic differentiation in mice. Biochem Biophys Res Commun 465(3) 638-43(2015) 26297947 |
Hosokawa M, Arai T, Masuda-Suzukake M, Kondo H, Matsuwaki T, Nishihara M, Hasegawa M, Akiyama H. Progranulin reduction is associated with increased tau phosphorylation in P301L tau transgenic mice. J Neuropathol Exp Neurol 74(2) 158-65(2015) 25575133 |
Hafler BP, Klein ZA, Jimmy Zhou Z, Strittmatter SM. Progressive retinal degeneration and accumulation of autofluorescent lipopigments in Progranulin deficient mice. Brain Res 1588 168-74(2014) 25234724 |
Kawase R, Ohama T, Matsuyama A, Matsuwaki T, Okada T, Yamashita T, Yuasa-Kawase M, Nakaoka H, Nakatani K, Inagaki M, Tsubakio-Yamamoto K, Masuda D, Nakagawa-Toyama Y, Nishida M, Ohmoto Y, Nishihara M, Komuro I, Yamashita S. Deletion of progranulin exacerbates atherosclerosis in ApoE knockout mice. Cardiovasc Res 100(1) 125-33(2013) 23847387 |