Strain Data Sheet

RBRC02372

Strain Information

Image
BRC No.RBRC02372
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Lck-p19Arf)2
Former Common nameB6-Tg(Lck-p19<Arf>)2
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain development
Strain description
Colony maintenance
References
Thymocyte proliferation induced by pre-T cell receptor signaling is maintained through polycomb gene product Bmi-1-mediated Cdkn2a repression.
Miyazaki M, Miyazaki K, Itoi M, Katoh Y, Guo Y, Kanno R, Katoh-Fukui Y, Honda H, Amagai T, van Lohuizen M, Kawamoto H, Kanno M
Immunity, 28, 231-245 (2008). 18275833

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cdkn2a
MGI:104738
cyclin-dependent kinase inhibitor 2AUNCdkn2a mouse lck proximal promoter
GH Growth hormone polyA (human)UNGH

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell differentiation(MP:0002145)

  • decreased DN4 thymocyte number(MP:0010136)

  • decreased double-positive T cell number(MP:0005092)

  • decreased thymocyte number(MP:0000715)

  • increased gamma-delta T cell number(MP:0008346)
  • more 1 phenotypes
  • increased thymocyte apoptosis(MP:0009541)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse lck genomic DNA, mouse p19Arf cDNA, human growth hormone genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorMasaki Miyazaki (Hiroshima University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jul 2008

    BRC mice in Publications

    No Data